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CALHM1 Polyclonal antibody

CALHM1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 22042-1-AP

Print datasheet

Synonyms

CALHM1, FAM26C, Protein FAM26C

验证数据展示

  • WB analysis of mouse brain using 22042-1-AP

    WB analysis of mouse brain using 22042-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 22042-1-AP (CALHM1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human brain using 22042-1-AP

    IHC staining of human brain using 22042-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 22042-1-AP (CALHM1 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human brain using 22042-1-AP

    IHC staining of human brain using 22042-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 22042-1-AP (CALHM1 antibody) at dilution of 1:50 (under 40x lens).

经过测试的应用

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

22042-1-AP targets CALHM1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen CALHM1 fusion protein Ag14938 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name calcium homeostasis modulator 1
Synonyms CALHM1, FAM26C, Protein FAM26C
Calculated Molecular Weight 346 aa, 38 kDa
Observed Molecular Weight 38-40 kDa
GenBank Accession NumberBC036193
Gene Symbol CALHM1
Gene ID (NCBI) 255022
RRIDAB_11182709
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8IU99
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Calcium homeostasis modulator 1 (CALHM1) is a membrane protein with four transmembrane helices that form an octameric ion channel with voltage-dependent activation.

实验方案

Product Specific Protocols
WB protocol for CALHM1 antibody 22042-1-APDownload protocol
IHC protocol for CALHM1 antibody 22042-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols