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CALHM2 Polyclonal antibody

CALHM2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 19931-1-AP

Print datasheet

Synonyms

CALHM2, FAM26B, Protein FAM26B

验证数据展示

  • WB analysis using 19931-1-AP

    WB analysis using 19931-1-AP

    Mouse brain extracted membrane protein and total protein lysates were subjected to SDS PAGE followed by western blot with 19931-1-AP (CALHM2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IF Staining of U2OS using 19931-1-AP

    IF Staining of U2OS using 19931-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using CALHM2 antibody (19931-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • IHC staining of human placenta using 19931-1-AP

    IHC staining of human placenta using 19931-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 19931-1-AP (CALHM2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inmouse brain tissue
Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU2OS cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

19931-1-AP targets CALHM2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB, IF
Tested Reactivity human, mouse, rat
Cited Reactivitymouse, rat
Immunogen CALHM2 fusion protein Ag13909 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name calcium homeostasis modulator 2
Synonyms CALHM2, FAM26B, Protein FAM26B
Calculated Molecular Weight 323 aa, 36 kDa
Observed Molecular Weight 32-36 kDa
GenBank Accession NumberBC000039
Gene Symbol CALHM2
Gene ID (NCBI) 51063
RRIDAB_2878626
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9HA72
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CALHM2, also named as FAM26B, is a pore-forming subunit of a voltage-gated ion channel. It's a critical ATP-releasing channel that modulates neural activity and as a potential risk factor of depression. There're three isoforms of CALHM2 with calculated MW 36 kDa, 24 kDa and 22 kDa. It's about 32-36 kDa in western blot with membrane protein extraceted lysate.

实验方案

Product Specific Protocols
WB protocol for CALHM2 antibody 19931-1-APDownload protocol
IHC protocol for CALHM2 antibody 19931-1-APDownload protocol
IF protocol for CALHM2 antibody 19931-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouse,ratWB

Cereb Cortex

P2X7 receptor-mediated depression-like reactions arising in the mouse medial prefrontal cortex

Authors - Wen-Jing Ren
View Article
mouseIF

Brain Res Bull

Upregulation of Calhm2 in the anterior cingulate cortex contributes to the maintenance of bilateral mechanical allodynia and comorbid anxiety symptoms in inflammatory pain conditions

Authors - Xiaohui Li
View Article