Calmodulin Polyclonal antibody
Calmodulin Polyclonal Antibody for WB, IHC, FC (Intra), IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (1)
Applications
WB, IHC, FC (Intra), IP, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | mouse skeletal muscle tissue, HepG2 cells, mouse brain tissue, MCF-7 cells, HeLa cells, NCCIT cells, rat skeletal muscle tissue |
Positive IP detected in | Ramos cells |
Positive IHC detected in | human breast cancer tissue, mouse testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive FC (Intra) detected in | HeLa cells, MCF-7 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
10541-1-AP targets Calmodulin in WB, IHC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | WB, IHC, FC (Intra), IP, ELISA Application Description |
Cited Applications | WB, IHC, IP |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat, bovine |
Immunogen | Calmodulin fusion protein Ag0827 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | calmodulin 3 (phosphorylase kinase, delta) |
Synonyms | Calmodulin 3, Calmodulin 1/2/3, CALML2, CALM3, CAM3 |
Calculated Molecular Weight | 17 kDa |
Observed Molecular Weight | 17 kDa |
GenBank Accession Number | BC006182 |
Gene Symbol | Calmodulin 3 |
Gene ID (NCBI) | 808 |
RRID | AB_2069442 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | P62158 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for Calmodulin antibody 10541-1-AP | Download protocol |
IHC protocol for Calmodulin antibody 10541-1-AP | Download protocol |
IP protocol for Calmodulin antibody 10541-1-AP | Download protocol |
FC protocol for Calmodulin antibody 10541-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Nat Commun Stalled translation by mitochondrial stress upregulates a CNOT4-ZNF598 ribosomal quality control pathway important for tissue homeostasis | ||
Autophagy Periplocin suppresses the growth of colorectal cancer cells by triggering LGALS3 (galectin 3)-mediated lysophagy | ||
Cancer Cell Int CALM1 promotes progression and dampens chemosensitivity to EGFR inhibitor in esophageal squamous cell carcinoma. | ||
Neurochem Int Alpha-synuclein overexpression increases phospho-protein phosphatase 2A levels via formation of calmodulin/Src complex. | ||
Biochem Biophys Res Commun The NMDAR GluN1-1a C-terminus binds to CaM and regulates synaptic function. | ||
J Cardiovasc Transl Res TRPC1 Deficiency Impairs the Endothelial Progenitor Cell Function via Inhibition of Calmodulin/eNOS Pathway. |