Calmodulin Polyclonal antibody

Calmodulin Polyclonal Antibody for WB, IHC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, FC (Intra), IP, ELISA

Conjugate

Unconjugated

Cat no : 10541-1-AP

Print datasheet

Synonyms

Calmodulin 3, Calmodulin 1/2/3, CALML2, CALM3, CAM3



经过测试的应用

Positive WB detected inmouse skeletal muscle tissue, HepG2 cells, mouse brain tissue, MCF-7 cells, HeLa cells, NCCIT cells, rat skeletal muscle tissue
Positive IP detected inRamos cells
Positive IHC detected inhuman breast cancer tissue, mouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inHeLa cells, MCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy CALM3 Ready-To-Use IHC Kit. CALM3 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10541-1-AP targets Calmodulin in WB, IHC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, FC (Intra), IP, ELISA Application Description
Cited ApplicationsWB, IHC, IP
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, bovine
Immunogen Calmodulin fusion protein Ag0827 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name calmodulin 3 (phosphorylase kinase, delta)
Synonyms Calmodulin 3, Calmodulin 1/2/3, CALML2, CALM3, CAM3
Calculated Molecular Weight 17 kDa
Observed Molecular Weight 17 kDa
GenBank Accession NumberBC006182
Gene Symbol Calmodulin 3
Gene ID (NCBI) 808
RRIDAB_2069442
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP62158
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Calmodulin (CaM) is a Ca(2+)-binding protein that transduces Ca2+-mediated signals by binding to and regulating the activity of hundreds of enzymes and non-enzymatic proteins. It is highly conserved across species and involved in many biological processes, including vesicle release, cell proliferation, and apoptosis. This antibody can recognize CALM1, CALM2 and CALM3.

实验方案

Product Specific Protocols
WB protocol for Calmodulin antibody 10541-1-APDownload protocol
IHC protocol for Calmodulin antibody 10541-1-APDownload protocol
IP protocol for Calmodulin antibody 10541-1-APDownload protocol
FC protocol for Calmodulin antibody 10541-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Nat Commun

Stalled translation by mitochondrial stress upregulates a CNOT4-ZNF598 ribosomal quality control pathway important for tissue homeostasis

Authors - Ji Geng
human

Autophagy

Periplocin suppresses the growth of colorectal cancer cells by triggering LGALS3 (galectin 3)-mediated lysophagy

Authors - Kui Wang
humanIHC, WB

Cancer Cell Int

CALM1 promotes progression and dampens chemosensitivity to EGFR inhibitor in esophageal squamous cell carcinoma.

Authors - Tao Liu
IP

Neurochem Int

Alpha-synuclein overexpression increases phospho-protein phosphatase 2A levels via formation of calmodulin/Src complex.

Authors - W Yang
humanWB

Biochem Biophys Res Commun

The NMDAR GluN1-1a C-terminus binds to CaM and regulates synaptic function.

Authors - Liang Zhou
humanWB

J Cardiovasc Transl Res

TRPC1 Deficiency Impairs the Endothelial Progenitor Cell Function via Inhibition of Calmodulin/eNOS Pathway.

Authors - Lai-Ling Du