验证数据展示
经过测试的应用
Positive WB detected in | mouse skeletal muscle tissue, HepG2 cells, mouse brain tissue, MCF-7 cells, HeLa cells, NCCIT cells, rat skeletal muscle tissue |
Positive IP detected in | Ramos cells |
Positive IHC detected in | human breast cancer tissue, mouse testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive FC (Intra) detected in | HeLa cells, MCF-7 cells |
推荐稀释比
应用 | 推荐稀释比 |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
10541-1-AP targets Calmodulin in WB, IHC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
经测试应用 | WB, IHC, FC (Intra), IP, ELISA Application Description |
文献引用应用 | WB, IHC, IP, CoIP |
经测试反应性 | human, mouse, rat |
文献引用反应性 | human, mouse, rat, bovine |
免疫原 | Calmodulin fusion protein Ag0827 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | calmodulin 3 (phosphorylase kinase, delta) |
别名 | Calmodulin 3, Calmodulin 1/2/3, CALML2, CALM3, CAM3 |
计算分子量 | 17 kDa |
观测分子量 | 17 kDa |
GenBank蛋白编号 | BC006182 |
基因名称 | Calmodulin 3 |
Gene ID (NCBI) | 808 |
RRID | AB_2069442 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | P62158 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Calmodulin (CaM) is a Ca(2+)-binding protein that transduces Ca2+-mediated signals by binding to and regulating the activity of hundreds of enzymes and non-enzymatic proteins. It is highly conserved across species and involved in many biological processes, including vesicle release, cell proliferation, and apoptosis. This antibody can recognize CALM1, CALM2 and CALM3.
实验方案
Product Specific Protocols | |
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WB protocol for Calmodulin antibody 10541-1-AP | Download protocol |
IHC protocol for Calmodulin antibody 10541-1-AP | Download protocol |
IP protocol for Calmodulin antibody 10541-1-AP | Download protocol |
FC protocol for Calmodulin antibody 10541-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Nat Commun Stalled translation by mitochondrial stress upregulates a CNOT4-ZNF598 ribosomal quality control pathway important for tissue homeostasis | ||
Nat Commun Selectively targeting the AdipoR2-CaM-CaMKII-NOS3 axis by SCM-198 as a rapid-acting therapy for advanced acute liver failure | ||
Autophagy Periplocin suppresses the growth of colorectal cancer cells by triggering LGALS3 (galectin 3)-mediated lysophagy | ||
Cancer Cell Int CALM1 promotes progression and dampens chemosensitivity to EGFR inhibitor in esophageal squamous cell carcinoma. | ||
Neurochem Int Alpha-synuclein overexpression increases phospho-protein phosphatase 2A levels via formation of calmodulin/Src complex. | ||
Biochem Biophys Res Commun The NMDAR GluN1-1a C-terminus binds to CaM and regulates synaptic function. |