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验证数据展示

  • IHC staining of human skin cancer using 13059-1-AP

    IHC staining of human skin cancer using 13059-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 13059-1-AP (CALML5 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human skin cancer using 13059-1-AP

    IHC staining of human skin cancer using 13059-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 13059-1-AP (CALML5 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

CALML5 Polyclonal antibody

CALML5 Polyclonal Antibody for IHC, ELISA
Cat No. 13059-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IHC, ELISA and More (1)

别名

CALML5, calmodulin like 5, Calmodulin like protein 5, Calmodulin like skin protein, CLSP

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive IHC detected inhuman skin cancer tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:300-1:1200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13059-1-AP targets CALML5 in IF, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 IHC, ELISA Application Description
文献引用应用IF, IHC
经测试反应性 human
文献引用反应性human
免疫原 CALML5 fusion protein Ag3734 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 calmodulin-like 5
别名 CALML5, calmodulin like 5, Calmodulin like protein 5, Calmodulin like skin protein, CLSP
计算分子量 146 aa, 16 kDa
GenBank蛋白编号BC039172
基因名称 CALML5
Gene ID (NCBI) 51806
RRIDAB_2069463
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NZT1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CALML5, also known as calmodulin-like skin protein (CLSP), a recently discovered skin-specific calcium-binding protein, is closely related to keratinocyte differentiation. Abundant expression is detected only in reconstructed epidermis and is restricted to differentiating keratinocytes. In addition, it can associate with transglutaminase 3, shown to be a key enzyme in the terminal differentiation of keratinocytes.

实验方案

Product Specific Protocols
IHC protocol for CALML5 antibody 13059-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC,IF

Ann Diagn Pathol

Loss of ELF3 immunoexpression is useful for detecting oral squamous cell carcinoma but not for distinguishing between grades of epithelial dysplasia.

Authors - Abdulmajeed Ahmad A AA
View Article
humanIHC

Elife

Dalpiciclib partially abrogates ER signaling activation induced by pyrotinib in HER2+HR+ breast cancer

Authors - Jiawen Bu
View Article