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CALR3 Polyclonal antibody

CALR3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 17064-1-AP

Print datasheet

Synonyms

CALR3, Calreticulin 2, calreticulin 3, Calreticulin-3, Calsperin, CRT2

验证数据展示

  • WB analysis of mouse testis using 17064-1-AP

    WB analysis of mouse testis using 17064-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 17064-1-AP (CALR3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human testis using 17064-1-AP

    IHC staining of human testis using 17064-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 17064-1-AP (CALR3 Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human testis using 17064-1-AP

    IHC staining of human testis using 17064-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 17064-1-AP (CALR3 Antibody) at dilution of 1:50 (under 40x lens).

经过测试的应用

Positive WB detected inmouse testis tissue
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17064-1-AP targets CALR3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen CALR3 fusion protein Ag10847 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name calreticulin 3
Synonyms CALR3, Calreticulin 2, calreticulin 3, Calreticulin-3, Calsperin, CRT2
Calculated Molecular Weight 384 aa, 45 kDa
Observed Molecular Weight 45 kDa
GenBank Accession NumberBC014595
Gene Symbol CALR3
Gene ID (NCBI) 125972
RRIDAB_2243959
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96L12
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for CALR3 antibody 17064-1-APDownload protocol
IHC protocol for CALR3 antibody 17064-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols