• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • IHC staining of human colon cancer using 24312-1-AP

    IHC staining of human colon cancer using 24312-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 24312-1-AP (CAMK1 antibody) at dilution of 1:200 (under 10x lens) Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0).

    Ready-to-use IHC kits available with CAMK1 antibody included
  • IHC staining of human colon cancer using 24312-1-AP

    IHC staining of human colon cancer using 24312-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 24312-1-AP (CAMK1 antibody) at dilution of 1:200 (under 40x lens) Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0).

    Ready-to-use IHC kits available with CAMK1 antibody included
  • WB analysis using 24312-1-AP

    WB analysis using 24312-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 24312-1-AP (CAMK1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IF Staining of HEK-293 using 24312-1-AP

    IF Staining of HEK-293 using 24312-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using 24312-1-AP (CAMK1 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • IP experiment of HEK-293 using 24312-1-AP

    IP experiment of HEK-293 using 24312-1-AP

    IP result of anti-CAMK1 (IP:24312-1-AP, 4ug; Detection:24312-1-AP 1:300) with HEK-293 cells lysate 3400ug.

CAMK1 Polyclonal antibody

CAMK1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 24312-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human and More (1)

应用

WB, IHC, IF/ICC, IP, ELISA

别名

CaM KI, CaM kinase I, CaM kinase I alpha, CAMK1, CAMKI, CaMKI alpha

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inHEK-293 cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman colon cancer tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHEK-293 cells
Planning an IHC experiment? We recommend our IHCeasy CAMK1 Ready-To-Use IHC Kit. CAMK1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24312-1-AP targets CAMK1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
文献引用应用WB, IHC
经测试反应性 human
文献引用反应性human, mouse
免疫原 CAMK1 fusion protein Ag19449 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 calcium/calmodulin-dependent protein kinase I
别名 CaM KI, CaM kinase I, CaM kinase I alpha, CAMK1, CAMKI, CaMKI alpha
计算分子量 370 aa, 41 kDa
观测分子量 41 kDa
GenBank蛋白编号BC106755
基因名称 CAMK1
Gene ID (NCBI) 8536
RRIDAB_2879489
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ14012
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for CAMK1 antibody 24312-1-APDownload protocol
IHC protocol for CAMK1 antibody 24312-1-APDownload protocol
IF protocol for CAMK1 antibody 24312-1-APDownload protocol
IP protocol for CAMK1 antibody 24312-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Biomed Res Int

Effect of Lentivirus-Mediated miR-499a-3p on Human Umbilical Vein Endothelial Cells.

Authors - Huilei Zheng
View Article
mouseIHC

Biochim Biophys Acta Mol Basis Dis

IGF2BP3-stabilized CAMK1 regulates the mitochondrial dynamics of renal tubule to alleviate diabetic nephropathy

Authors - Du Yuan
View Article