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CAMK2 Monoclonal antibody, PBS Only

CAMK2 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat, Pig

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1E3B3

Cat no : 66843-1-PBS

Print datasheet

Synonyms

CaM kinase II subunit alpha, CaMK II subunit alpha, CAMK2A, CAMK2B, CAMK2D, CAMK2G, CAMKA, KIAA0968

验证数据展示

  • IF Staining of SH-SY5Y using 66843-1-Ig (same clone as 66843-1-PBS)

    IF Staining of SH-SY5Y using 66843-1-Ig (same clone as 66843-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using CAMK2 antibody (66843-1-Ig, Clone: 1E3B3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66843-1-PBS in a different storage buffer formulation.

  • WB analysis using 66843-1-Ig (same clone as 66843-1-PBS)

    WB analysis using 66843-1-Ig (same clone as 66843-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66843-1-Ig (CAMK2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66843-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 66843-1-Ig (same clone as 66843-1-PBS)

    IHC staining of mouse brain using 66843-1-Ig (same clone as 66843-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66843-1-Ig (CAMK2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66843-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 66843-1-Ig (same clone as 66843-1-PBS)

    IHC staining of mouse brain using 66843-1-Ig (same clone as 66843-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66843-1-Ig (CAMK2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66843-1-PBS in a different storage buffer formulation.

  • WB analysis using 66843-1-Ig (same clone as 66843-1-PBS)

    WB analysis using 66843-1-Ig (same clone as 66843-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66843-1-Ig (CAMK2A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66843-1-PBS in a different storage buffer formulation.

产品信息

66843-1-PBS targets CAMK2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, Mouse, Rat, Pig samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity Human, Mouse, Rat, Pig
Immunogen CAMK2 fusion protein Ag4736 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name calcium/calmodulin-dependent protein kinase II alpha
Synonyms CaM kinase II subunit alpha, CaMK II subunit alpha, CAMK2A, CAMK2B, CAMK2D, CAMK2G, CAMKA, KIAA0968
Calculated Molecular Weight 478 aa, 54 kDa
Observed Molecular Weight 45-60 kDa
GenBank Accession NumberBC040457
Gene Symbol CaMKII alpha
Gene ID (NCBI) 815
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9UQM7
Storage Buffer PBS only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CaM-kinase II (CAMK2) is a prominent kinase in the central nervous system that may function in long-term potentiation and neurotransmitter release. It is a member of the NMDAR signaling complex in excitatory synapses it may regulate NMDAR-dependent potentiation of the AMPAR and synaptic plasticity. This antibody can recognize all the members of CAMK2 family.