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验证数据展示

  • WB analysis of mouse brain using 55140-1-AP

    WB analysis of mouse brain using 55140-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 55140-1-AP (CaMKII gamma-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human skeletal muscle using 55140-1-AP

    IHC staining of human skeletal muscle using 55140-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 55140-1-AP (CaMKII gamma-Specific antibody) at dilution of 1:50 (under 10x lens).

    Ready-to-use IHC kits available with CAMK2G antibody included
  • IHC staining of human skeletal muscle using 55140-1-AP

    IHC staining of human skeletal muscle using 55140-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 55140-1-AP (CaMKII gamma-Specific antibody) at dilution of 1:50 (under 40x lens).

    Ready-to-use IHC kits available with CAMK2G antibody included

CaMKII gamma-Specific Polyclonal antibody

CaMKII gamma-Specific Polyclonal Antibody for WB, IHC, ELISA
Cat No. 55140-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

CaM kinase II subunit gamma, CAMK, CAMK II, CaMK II subunit gamma, CAMK2G, CAMKG, CaMKII gamma

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse brain tissue
Positive IHC detected inhuman skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy CAMK2G Ready-To-Use IHC Kit. CAMK2G primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

55140-1-AP targets CaMKII gamma-Specific in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性human
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 calcium/calmodulin-dependent protein kinase II gamma
别名 CaM kinase II subunit gamma, CAMK, CAMK II, CaMK II subunit gamma, CAMK2G, CAMKG, CaMKII gamma
计算分子量 65 kDa
观测分子量 45-65 kDa
GenBank蛋白编号NM_001222
基因名称 CAMK2G
Gene ID (NCBI) 818
RRIDAB_10858395
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ13555
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Aliquoting is unnecessary for -20oC storage.

背景介绍

CaMKII gamma, also named as CAMKG, belongs to the protein kinase superfamily, CAMK Ser/Thr protein kinase family and CaMK subfamily. It is a prominent kinase in the central nervous system that may function in long-term potentiation and neurotransmitter release. CaMK2 is a Ca2+-signaling intermediate that contains α, β, γ and δ subunits. The antibody is specific to CAMK2G.

实验方案

Product Specific Protocols
WB protocol for CaMKII gamma-Specific antibody 55140-1-APDownload protocol
IHC protocol for CaMKII gamma-Specific antibody 55140-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Aging (Albany NY)

Membrane-cytoplasm translocation of annexin A4 is involved in the metastasis of colorectal carcinoma.

Authors - Ya Peng
View Article
humanWB

Biomed Pharmacother

Novel synthetic tosyl chloride-berbamine regresses lethal MYC-positive leukemia by targeting CaMKIIγ/Myc axis.

Authors - Qingfeng Yu
View Article