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验证数据展示

  • WB analysis using 68519-1-Ig

    WB analysis using 68519-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68519-1-Ig (CAMK2G antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis using 68519-1-Ig

    WB analysis using 68519-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68519-1-Ig (CAMK2G antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

CAMK2G Monoclonal antibody

CAMK2G Monoclonal Antibody for WB, ELISA
Cat No. 68519-1-Ig

产品说明书

CloneNo. 1C12H10

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat, pig, rabbit

应用

WB, ELISA

别名

CaMKII gamma, CAMK-II, CAMK, CaM kinase II subunit gamma, Calcium/calmodulin-dependent protein kinase type II subunit gamma

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inPC-3 cells, pig brain tissue, LNCaP cells, HCT 116 cells, rabbit brain tissue, rat brain tissue, mouse brain tissue
Planning an IHC experiment? We recommend our IHCeasy CAMK2G Ready-To-Use IHC Kit. CAMK2G primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68519-1-Ig targets CAMK2G in WB, ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human, mouse, rat, pig, rabbit
免疫原 CAMK2G fusion protein Ag23180 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 calcium/calmodulin-dependent protein kinase II gamma
别名 CaMKII gamma, CAMK-II, CAMK, CaM kinase II subunit gamma, Calcium/calmodulin-dependent protein kinase type II subunit gamma
计算分子量 527 aa, 59 kDa
观测分子量64 kDa
GenBank蛋白编号BC034044
基因名称 CAMK2G
Gene ID (NCBI) 818
RRIDAB_3085228
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ13555
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CaMKII gamma, also named CAMKG or CAMK2G, belongs to the protein kinase superfamily, CAMK Ser/Thr protein kinase family, and CaMK subfamily. CAMK2 is a prominent kinase in the central nervous system that may function in long-term potentiation and neurotransmitter release. CAMK2G is expressed in human beta cells as a candidate gene for Type II (non-insulin-dependent) diabetes mellitus. Some studies can be used to assess the role of CAMK2G in the susceptibility to Type II diabetes(PMID: 12032636). CAMK2G has some isoforms with the MW of 55-66 kDa and this antibody can recognize all the isoforms of CAMK2G. This antibody may have cross-reaction with CAMK2A/B/D due to the high homology.

实验方案

Product Specific Protocols
WB protocol for CAMK2G antibody 68519-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols