CaMKIV Polyclonal antibody
CaMKIV Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | human cerebellum tissue, mouse thymus tissue |
| Positive IHC detected in | human ovary cancer tissue, human brain tissue, human ovary tumor tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2400 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 5 publications below |
| IF | See 1 publications below |
产品信息
13263-1-AP targets CaMKIV in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB, IHC, ELISA Application Description |
| Cited Applications | WB, IF |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Immunogen | CaMKIV fusion protein Ag3922 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | calcium/calmodulin-dependent protein kinase IV |
| Synonyms | CAMK4, CaMK IV, CaMK GR, CAMK, CaM kinase-GR |
| Calculated Molecular Weight | 473 aa, 52 kDa |
| Observed Molecular Weight | 56-65 kDa |
| GenBank Accession Number | BC025687 |
| Gene Symbol | CAMK4 |
| Gene ID (NCBI) | 814 |
| RRID | AB_2068381 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q16566 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CaMK4 (Calcium/calmodulin-dependent protein kinase IV) is a multifunctional serine/threonine kinase that regulates gene expression by activating transcription factors in a wide range of immune cells including T cells and antigen-presenting cells. The function of CaMK4 is suggested to be abnormal mainly in systemic lupus erythematosus (SLE), which is characterized by autoantibody production, immune complex formation, and immune dysregulation (PMID: 29252071). CaMKIV can translocate between the nucleus and cytoplasm (PMID: 21301225)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CaMKIV antibody 13263-1-AP | Download protocol |
| IHC protocol for CaMKIV antibody 13263-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Oncotarget PIWIL1 destabilizes microtubule by suppressing phosphorylation at Ser16 and RLIM-mediated degradation of Stathmin1. | ||
J Invest Dermatol The Long Noncoding RNA UCA1 Negatively Regulates Melanogenesis in Melanocytes. | ||
Toxicol Res (Camb) Neurotoxicity, behavioral changes and gene-expression profile of mice exposed to SnS2 nanoflowers. | ||
Cell Death Discov Congenital hypothyroidism impairs spine growth of dentate granule cells by downregulation of CaMKIV. | ||
Fitoterapia Investigation of the underlying mechanism of Buyang Huanwu decoction in ischemic stroke by integrating systems pharmacology-proteomics and in vivo experiments |
