验证数据展示
经过测试的应用
| Positive WB detected in | mouse brain tissue, HeLa cells, mouse spleen tissue, PC-3 cells |
| Positive IP detected in | mouse brain tissue |
| Positive IF-P detected in | mouse brain tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2400 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
14788-1-AP targets CAMKV in WB, IHC, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IF-P, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | CAMKV fusion protein Ag6438 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | CaM kinase-like vesicle-associated |
| 别名 | VACAMKL, CaM kinase-like vesicle-associated protein |
| 计算分子量 | 54 kDa |
| 观测分子量 | 54-75 kDa |
| GenBank蛋白编号 | BC000497 |
| 基因名称 | CAMKV |
| Gene ID (NCBI) | 79012 |
| RRID | AB_2068552 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q8NCB2 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CAMKV(CaM kinase-like vesicle-associated protein) is also named as 1G5,VACAMKL and belongs to the CAMK Ser/Thr protein kinase family. The CAMKV protein, which accumulates predominantly during postnatal development, binds to calmodulin in the presence of calcium. It is detected only in the CNS, is absent from cerebellum, but is detected in most other brain regions, especially regions of the cortex. The protein is predominantly detected in association with the plasma membrane of somatic cell and in neurites, both axons and dendrites, and is frequently observed in active zones, usually associated with vesicle-like structures. Its sequence suggests that lG5 may be membrane associated(PMID:8283228).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CAMKV antibody 14788-1-AP | Download protocol |
| IF protocol for CAMKV antibody 14788-1-AP | Download protocol |
| IP protocol for CAMKV antibody 14788-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Mol Neurodegener Nuclear speckle specific hnRNP D-like prevents age- and AD-related cognitive decline by modulating RNA splicing. | ||
Mol Oncol Ryanodine receptor 2 promotes colorectal cancer metastasis by the ROS/BACH1 axis | ||
Ann Neurol Paraneoplastic Calmodulin Kinase-Like Vesicle-Associated Protein (CAMKV) Autoimmune Encephalitis |
