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CAMLG Polyclonal antibody

CAMLG Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, WB,ELISA

Conjugate

Unconjugated

Cat No : 23327-1-AP

Print datasheet

Synonyms

calcium modulating ligand, CAML, CAMLG

验证数据展示

  • WB analysis of Jurkat using 23327-1-AP

    WB analysis of Jurkat using 23327-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 23327-1-AP (CAMLG antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 23327-1-AP

    IHC staining of human breast cancer using 23327-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 23327-1-AP (CAMLG Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human breast cancer using 23327-1-AP

    IHC staining of human breast cancer using 23327-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 23327-1-AP (CAMLG Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inJurkat cells, human testis tissue, mouse brain tissue, mouse testis tissue, rat brain tissue
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 23327-1-AP is CAMLG Fusion Protein expressed in E. coli.

Tested Applications IHC, WB,ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen CAMLG fusion protein Ag19915 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name calcium modulating ligand
Synonyms calcium modulating ligand, CAML, CAMLG
Calculated Molecular Weight 296 aa, 33 kDa
Observed Molecular Weight 36 kDa
GenBank Accession NumberBC130325
Gene Symbol CAMLG
Gene ID (NCBI) 819
RRIDAB_2879256
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP49069
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for CAMLG antibody 23327-1-APDownload protocol
IHC protocol for CAMLG antibody 23327-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols