验证数据展示
经过测试的应用
| Positive WB detected in | PC-3 cells, mouse skeletal muscle tissue, mouse testis tissue, mouse heart tissue, HepG2 cells, mouse kidney tissue, rat testis tissue, human kidney tissue |
| Positive IP detected in | mouse testis tissue |
| Positive IHC detected in | human liver cancer tissue, human skeletal muscle tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | U2OS cells |
Planning an IHC experiment? We recommend our IHCeasy CAP2 Ready-To-Use IHC Kit. CAP2 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
15865-1-AP targets CAP2 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, CoIP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | CAP2 fusion protein Ag8477 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | CAP, adenylate cyclase-associated protein, 2 (yeast) |
| 别名 | Adenylyl cyclase associated protein 2, CAP 2, CAP2 |
| 计算分子量 | 477 aa, 53 kDa |
| 观测分子量 | 53 kDa |
| GenBank蛋白编号 | BC008481 |
| 基因名称 | CAP2 |
| Gene ID (NCBI) | 10486 |
| RRID | AB_2270174 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P40123 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CAPs (CAP1 and CAP2) are evolutionarily conserved proteins with roles in regulating the actin cytoskeleton and in signal transduction. CAP2 is predominantly expressed in brain, heart and skeletal muscle, and skin. It is found in the nucleus in undifferentiated myoblasts and at the M-line of differentiated myotubes. Overexpression of CAP2 has been reported in many cancers, including hepatocellular carcinoma (HCC), human breast cancer, and malignant melanoma.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CAP2 antibody 15865-1-AP | Download protocol |
| IHC protocol for CAP2 antibody 15865-1-AP | Download protocol |
| IF protocol for CAP2 antibody 15865-1-AP | Download protocol |
| IP protocol for CAP2 antibody 15865-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Clin Invest CAP2 promotes gastric cancer metastasis by mediating the interaction between tumor cells and tumor-associated macrophages
| ||
Int J Mol Sci Analysis of mRNA and Protein Levels of CAP2, DLG1 and ADAM10 Genes in Post-Mortem Brain of Schizophrenia, Parkinson's and Alzheimer's Disease Patients. | ||
Commun Biol CAP2 is a regulator of actin pointed end dynamics and myofibrillogenesis in cardiac muscle.
| ||
Sci Rep Cyclase-associated protein 2 (CAP2) controls MRTF-A localization and SRF activity in mouse embryonic fibroblasts. | ||
Oncol Rep Expression status of cyclase‑associated protein 2 as a prognostic marker for human breast cancer. | ||
Sci Signal The actin-binding protein CAP1 represses MRTF-SRF-dependent gene expression in mouse cerebral cortex |
