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验证数据展示

  • WB analysis using 67412-1-Ig

    WB analysis using 67412-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67412-1-Ig (CAP2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • WB analysis of HepG2 using 67412-1-Ig

    WB analysis of HepG2 using 67412-1-Ig

    HepG2 cells were subjected to SDS PAGE followed by western blot with 67412-1-Ig (CAP2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

CAP2 Monoclonal antibody

CAP2 Monoclonal Antibody for WB, ELISA
Cat No. 67412-1-Ig

Print datasheet

CloneNo. 1G7D2

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

WB, ELISA

Synonyms

CAP 2, CAP2

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inA549 cells, HeLa cells, HepG2 cells, PC-3 cells, COLO 320 cells
Planning an IHC experiment? We recommend our IHCeasy CAP2 Ready-To-Use IHC Kit. CAP2 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67412-1-Ig targets CAP2 in WB, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity Human
Immunogen CAP2 fusion protein Ag8622 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name CAP, adenylate cyclase-associated protein, 2 (yeast)
Synonyms CAP 2, CAP2
Calculated Molecular Weight 477 aa, 53 kDa
Observed Molecular Weight 51-53 kDa
GenBank Accession NumberBC008481
Gene Symbol CAP2
Gene ID (NCBI) 10486
RRIDAB_2882653
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP40123
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CAPs (CAP1 and CAP2) are evolutionarily conserved proteins with roles in regulating the actin cytoskeleton and in signal transduction. CAP2 is predominantly expressed in brain, heart and skeletal muscle, and skin. It is found in the nucleus in undifferentiated myoblasts and at the M-line of differentiated myotubes. Overexpression of CAP2 has been reported in many cancers, including hepatocellular carcinoma (HCC), human breast cancer, and malignant melanoma.

实验方案

Product Specific Protocols
WB protocol for CAP2 antibody 67412-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols