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验证数据展示

  • WB analysis using 67412-1-Ig

    WB analysis using 67412-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67412-1-Ig (CAP2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • WB analysis of HepG2 using 67412-1-Ig

    WB analysis of HepG2 using 67412-1-Ig

    HepG2 cells were subjected to SDS PAGE followed by western blot with 67412-1-Ig (CAP2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

CAP2 Monoclonal antibody

CAP2 Monoclonal Antibody for WB, ELISA
Cat No. 67412-1-Ig

产品说明书

CloneNo. 1G7D2

宿主/亚型

Mouse / IgG2a

种属反应性

Human

应用

WB, ELISA

别名

CAP 2, CAP2

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA549 cells, HeLa cells, HepG2 cells, PC-3 cells, COLO 320 cells
Planning an IHC experiment? We recommend our IHCeasy CAP2 Ready-To-Use IHC Kit. CAP2 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67412-1-Ig targets CAP2 in WB, ELISA applications and shows reactivity with Human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 Human
免疫原 CAP2 fusion protein Ag8622 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 CAP, adenylate cyclase-associated protein, 2 (yeast)
别名 CAP 2, CAP2
计算分子量 477 aa, 53 kDa
观测分子量 51-53 kDa
GenBank蛋白编号BC008481
基因名称 CAP2
Gene ID (NCBI) 10486
RRIDAB_2882653
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP40123
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CAPs (CAP1 and CAP2) are evolutionarily conserved proteins with roles in regulating the actin cytoskeleton and in signal transduction. CAP2 is predominantly expressed in brain, heart and skeletal muscle, and skin. It is found in the nucleus in undifferentiated myoblasts and at the M-line of differentiated myotubes. Overexpression of CAP2 has been reported in many cancers, including hepatocellular carcinoma (HCC), human breast cancer, and malignant melanoma.

实验方案

Product Specific Protocols
WB protocol for CAP2 antibody 67412-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols