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CAPN1 Monoclonal antibody

CAPN1 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human and More (2)

Applications

WB, IHC, ELISA and More (1)

Conjugate

Unconjugated

CloneNo.

4E6H1

Cat no : 67732-1-Ig

Print datasheet

Synonyms

Calpain 1 catalytic subunit, Calpain 1 large subunit, Calpain mu type, CANP, CANP 1, CANP1, CANPL1, CAPN1, Micromolar calpain, muCANP, muCL



经过测试的应用

Positive WB detected inA431 cells, HeLa cells, Jurkat cells, LNCaP cells, T-47D cells, K562 cells
Positive IHC detected inhuman urothelial carcinoma tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67732-1-Ig targets CAPN1 in WB, IF, IHC, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB, IF
Tested Reactivity Human
Cited Reactivityhuman, mouse
Immunogen CAPN1 fusion protein Ag30522 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name calpain 1, (mu/I) large subunit
Synonyms Calpain 1 catalytic subunit, Calpain 1 large subunit, Calpain mu type, CANP, CANP 1, CANP1, CANPL1, CAPN1, Micromolar calpain, muCANP, muCL
Calculated Molecular Weight 704 aa, 82 kDa
Observed Molecular Weight 75 kDa
GenBank Accession NumberBC008751
Gene Symbol CAPN1
Gene ID (NCBI) 823
RRIDAB_2918501
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP07384
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for CAPN1 antibody 67732-1-IgDownload protocol
IHC protocol for CAPN1 antibody 67732-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Oxid Med Cell Longev

Polysaccharides from Polygonatum cyrtonema Hua Reduce Depression-Like Behavior in Mice by Inhibiting Oxidative Stress-Calpain-1-NLRP3 Signaling Axis.

Authors - Fengming Shen
humanWB

Cell Rep

Epstein-Barr virus BNRF1 destabilizes SMC5/6 cohesin complexes to evade its restriction of replication compartments.

Authors - Stephanie Pei Tung Yiu
mouseWB,IF

Neural Plast

Astragaloside IV Alleviates Brain Injury Induced by Hypoxia via the Calpain-1 Signaling Pathway

Authors - Yan Meng