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CAPN1 Monoclonal antibody

CAPN1 Monoclonal Antibody for WB, IHC, ELISA
Cat No. 67732-1-Ig

产品说明书

CloneNo. 4E6H1

宿主/亚型

Mouse / IgG2b

种属反应性

Human and More (2)

应用

WB, IHC, ELISA and More (1)

Calpain 1 catalytic subunit, Calpain 1 large subunit, Calpain mu type, CANP, CANP 1, CANP1, CANPL1, CAPN1, Micromolar calpain, muCANP, muCL

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inA431 cells, HeLa cells, Jurkat cells, LNCaP cells, T-47D cells, K562 cells
Positive IHC detected inhuman urothelial carcinoma tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67732-1-Ig targets CAPN1 in WB, IF, IHC, ELISA applications and shows reactivity with Human samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB, IF
经测试反应性 Human
文献引用反应性human, mouse
免疫原 CAPN1 fusion protein Ag30522 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 calpain 1, (mu/I) large subunit
别名 Calpain 1 catalytic subunit, Calpain 1 large subunit, Calpain mu type, CANP, CANP 1, CANP1, CANPL1, CAPN1, Micromolar calpain, muCANP, muCL
计算分子量 704 aa, 82 kDa
观测分子量 75 kDa
GenBank蛋白编号BC008751
基因名称 CAPN1
Gene ID (NCBI) 823
RRIDAB_2918501
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP07384
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for CAPN1 antibody 67732-1-IgDownload protocol
IHC protocol for CAPN1 antibody 67732-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Oxid Med Cell Longev

Polysaccharides from Polygonatum cyrtonema Hua Reduce Depression-Like Behavior in Mice by Inhibiting Oxidative Stress-Calpain-1-NLRP3 Signaling Axis.

Authors - Fengming Shen
humanWB

Cell Rep

Epstein-Barr virus BNRF1 destabilizes SMC5/6 cohesin complexes to evade its restriction of replication compartments.

Authors - Stephanie Pei Tung Yiu
mouseWB,IF

Neural Plast

Astragaloside IV Alleviates Brain Injury Induced by Hypoxia via the Calpain-1 Signaling Pathway

Authors - Yan Meng
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