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验证数据展示

  • WB analysis of PC-3 using 13675-1-AP

    WB analysis of PC-3 using 13675-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 13675-1-AP (Calpain 11 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse testis using 13675-1-AP

    IHC staining of mouse testis using 13675-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 13675-1-AP (Calpain 11 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using 13675-1-AP

    IF Staining of HeLa using 13675-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Calpain 11 antibody (13675-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

Calpain 11 Polyclonal antibody

Calpain 11 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 13675-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

EC:3.4.22.-, CAPN11, CANP 11, Calpain-11, calpain11

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inPC-3 cells
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13675-1-AP targets Calpain 11 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 Calpain 11 fusion protein Ag4668 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 calpain 11
别名 EC:3.4.22.-, CAPN11, CANP 11, Calpain-11, calpain11
计算分子量 702 aa, 81 kDa
观测分子量 81 kDa
GenBank蛋白编号BC033733
基因名称 Calpain 11
Gene ID (NCBI) 11131
RRIDAB_2069480
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9UMQ6
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Calpains are a superfamily of related proteins, some of which have been shown to function as intracellular calcium-dependent cysteine proteases. Calpains consist of a variable large (80 kDa) subunit and an invariant small (30 kDa) subunit. The expression of calpain11 during spermatogenesis and its localization is spermatozoa suggest that it is involved in regulating calcium-dependent signal transduction events during meiosis and sperm functional processes(PMID:16541461). And the 110 kDa band protein may be regarded as a complex.

实验方案

Product Specific Protocols
WB protocol for Calpain 11 antibody 13675-1-APDownload protocol
IHC protocol for Calpain 11 antibody 13675-1-APDownload protocol
IF protocol for Calpain 11 antibody 13675-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols