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验证数据展示

  • IHC staining of mouse colon using 55371-1-AP

    IHC staining of mouse colon using 55371-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 55371-1-AP (CAPN13 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse colon using 55371-1-AP

    IHC staining of mouse colon using 55371-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 55371-1-AP (CAPN13 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

CAPN13 Polyclonal antibody

CAPN13 Polyclonal Antibody for IHC, ELISA
Cat No. 55371-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

IHC, ELISA

别名

CANP 13, calpain 13

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive IHC detected inmouse colon tissue, human colon tissue, mouse small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

55371-1-AP targets CAPN13 in IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 IHC, ELISA Application Description
经测试反应性 human, mouse
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 calpain 13
别名 CANP 13, calpain 13
计算分子量 77 kDa
GenBank蛋白编号NM_144575
基因名称 CAPN13
Gene ID (NCBI) 92291
RRIDAB_3086447
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ6MZZ7
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The calpains, calcium-activated neutral proteases, are nonlysosomal, intracellular cysteine proteases. The mammalian calpains include ubiquitous, stomach-specific, and muscle-specific proteins. The ubiquitous enzymes consist of heterodimers with distinct large, catalytic subunits associated with a common small, regulatory subunit.

实验方案

Product Specific Protocols
IHC protocol for CAPN13 antibody 55371-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols