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CAPN13 Polyclonal antibody

CAPN13 Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, ELISA

Conjugate

Unconjugated

Cat no : 55371-1-AP

Print datasheet

Synonyms

CANP 13, calpain 13

验证数据展示

  • IHC staining of mouse colon using 55371-1-AP

    IHC staining of mouse colon using 55371-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 55371-1-AP (CAPN13 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse colon using 55371-1-AP

    IHC staining of mouse colon using 55371-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 55371-1-AP (CAPN13 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive IHC detected inmouse colon tissue, human colon tissue, mouse small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

55371-1-AP targets CAPN13 in IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications IHC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name calpain 13
Synonyms CANP 13, calpain 13
Calculated Molecular Weight 77 kDa
GenBank Accession NumberNM_144575
Gene Symbol CAPN13
Gene ID (NCBI) 92291
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ6MZZ7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The calpains, calcium-activated neutral proteases, are nonlysosomal, intracellular cysteine proteases. The mammalian calpains include ubiquitous, stomach-specific, and muscle-specific proteins. The ubiquitous enzymes consist of heterodimers with distinct large, catalytic subunits associated with a common small, regulatory subunit.

实验方案

Product Specific Protocols
IHC protocol for CAPN13 antibody 55371-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols