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CAPN2 Monoclonal antibody, PBS Only

CAPN2 Monoclonal Antibody for WB,IHC,IF,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

WB,IHC,IF,ELISA

Conjugate

Unconjugated

CloneNo.

1E1F10

Cat No : 66977-1-PBS

Print datasheet

Synonyms

Calpain 2 catalytic subunit, Calpain 2 large subunit, Calpain large polypeptide L2, Calpain M type, CANP 2, CANP2, CANPL2, CANPml, CAPN2, FLJ39928, M calpain, mCANP, Millimolar calpain

验证数据展示

  • WB analysis using 66977-1-Ig (same clone as 66977-1-PBS)

    WB analysis using 66977-1-Ig (same clone as 66977-1-PBS)

    A549 cells were subjected to SDS PAGE followed by western blot with 66977-1-Ig (CAPN2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66977-1-PBS in a different storage buffer formulation.

  • IHC staining of human pancreas cancer using 66977-1-Ig (same clone as 66977-1-PBS)

    IHC staining of human pancreas cancer using 66977-1-Ig (same clone as 66977-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66977-1-Ig (CAPN2 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66977-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CAPN2 antibody included
  • IF Staining of HepG2 using 66977-1-Ig (same clone as 66977-1-PBS)

    IF Staining of HepG2 using 66977-1-Ig (same clone as 66977-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using CAPN2 antibody (66977-1-Ig, Clone: 1E1F10 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66977-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 66977-1-Ig (same clone as 66977-1-PBS)

    IHC staining of human colon cancer using 66977-1-Ig (same clone as 66977-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66977-1-Ig (CAPN2 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66977-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CAPN2 antibody included
  • IHC staining of human stomach cancer using 66977-1-Ig (same clone as 66977-1-PBS)

    IHC staining of human stomach cancer using 66977-1-Ig (same clone as 66977-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 66977-1-Ig (CAPN2 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66977-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CAPN2 antibody included
  • IHC staining of rat colon using 66977-1-Ig (same clone as 66977-1-PBS)

    IHC staining of rat colon using 66977-1-Ig (same clone as 66977-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat small intestine tissue slide using 66977-1-Ig (CAPN2 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66977-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CAPN2 antibody included
  • WB analysis using 66977-1-Ig (same clone as 66977-1-PBS)

    WB analysis using 66977-1-Ig (same clone as 66977-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66977-1-Ig (CAPN2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66977-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy CAPN2 Ready-To-Use IHC Kit. CAPN2 primary antibody included.

产品信息

66977-1-PBS targets CAPN2 in WB, IF, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Applications WB,IHC,IF,ELISA Application Description
Tested Reactivity Human, Mouse, Rat
Immunogen CAPN2 fusion protein Ag28427 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name calpain 2, (m/II) large subunit
Synonyms Calpain 2 catalytic subunit, Calpain 2 large subunit, Calpain large polypeptide L2, Calpain M type, CANP 2, CANP2, CANPL2, CANPml, CAPN2, FLJ39928, M calpain, mCANP, Millimolar calpain
Calculated Molecular Weight 700 aa, 80 kDa
Observed Molecular Weight 72-80 kDa
GenBank Accession NumberBC021303
Gene Symbol CAPN2
Gene ID (NCBI) 824
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP17655
Storage Buffer PBS only
Storage ConditionsStore at -80°C.

背景介绍

Calpain 2(Calpain-2 catalytic subunit) is also named as CANPL2, CANPml, mCANP, FLJ39928 and belongs to the peptidase C2 family. N-terminal sequencing of CAPN2 purified from human liver indicates that the N-terminal methionine is removed, resulting in a mature 699-amino acid subunit with a calculated molecular mass of 79.9 kD(PMID:2852952). It is a calcium-regulated non-lysosomal thiol-protease which catalyzes limited proteolysis of substrates involved in cytoskeletal remodelling and signal transduction. It has 2 isoforms produced by alternative splicing with the molecular weight of 80 kDa and 71 kDa.