• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

CAPN2 Monoclonal antibody, PBS Only

CAPN2 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1E1F10

Cat no : 66977-1-PBS

Print datasheet

Synonyms

Calpain 2 catalytic subunit, Calpain 2 large subunit, Calpain large polypeptide L2, Calpain M type, CANP 2, CANP2, CANPL2, CANPml, CAPN2, FLJ39928, M calpain, mCANP, Millimolar calpain

验证数据展示

  • WB analysis using 66977-1-Ig (same clone as 66977-1-PBS)

    WB analysis using 66977-1-Ig (same clone as 66977-1-PBS)

    A549 cells were subjected to SDS PAGE followed by western blot with 66977-1-Ig (CAPN2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66977-1-PBS in a different storage buffer formulation.

  • IHC staining of human pancreas cancer using 66977-1-Ig (same clone as 66977-1-PBS)

    IHC staining of human pancreas cancer using 66977-1-Ig (same clone as 66977-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66977-1-Ig (CAPN2 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66977-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CAPN2 antibody included
  • IF Staining of HepG2 using 66977-1-Ig (same clone as 66977-1-PBS)

    IF Staining of HepG2 using 66977-1-Ig (same clone as 66977-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using CAPN2 antibody (66977-1-Ig, Clone: 1E1F10 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66977-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 66977-1-Ig (same clone as 66977-1-PBS)

    IHC staining of human colon cancer using 66977-1-Ig (same clone as 66977-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66977-1-Ig (CAPN2 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66977-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CAPN2 antibody included
  • IHC staining of human stomach cancer using 66977-1-Ig (same clone as 66977-1-PBS)

    IHC staining of human stomach cancer using 66977-1-Ig (same clone as 66977-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 66977-1-Ig (CAPN2 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66977-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CAPN2 antibody included
  • IHC staining of rat colon using 66977-1-Ig (same clone as 66977-1-PBS)

    IHC staining of rat colon using 66977-1-Ig (same clone as 66977-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat small intestine tissue slide using 66977-1-Ig (CAPN2 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66977-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CAPN2 antibody included
  • WB analysis using 66977-1-Ig (same clone as 66977-1-PBS)

    WB analysis using 66977-1-Ig (same clone as 66977-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66977-1-Ig (CAPN2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66977-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy CAPN2 Ready-To-Use IHC Kit. CAPN2 primary antibody included.

产品信息

66977-1-PBS targets CAPN2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity Human, Mouse, Rat
Immunogen CAPN2 fusion protein Ag28427 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name calpain 2, (m/II) large subunit
Synonyms Calpain 2 catalytic subunit, Calpain 2 large subunit, Calpain large polypeptide L2, Calpain M type, CANP 2, CANP2, CANPL2, CANPml, CAPN2, FLJ39928, M calpain, mCANP, Millimolar calpain
Calculated Molecular Weight 700 aa, 80 kDa
Observed Molecular Weight 72-80 kDa
GenBank Accession NumberBC021303
Gene Symbol CAPN2
Gene ID (NCBI) 824
RRIDAB_2882297
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP17655
Storage Buffer PBS only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Calpain 2(Calpain-2 catalytic subunit) is also named as CANPL2, CANPml, mCANP, FLJ39928 and belongs to the peptidase C2 family. N-terminal sequencing of CAPN2 purified from human liver indicates that the N-terminal methionine is removed, resulting in a mature 699-amino acid subunit with a calculated molecular mass of 79.9 kD(PMID:2852952). It is a calcium-regulated non-lysosomal thiol-protease which catalyzes limited proteolysis of substrates involved in cytoskeletal remodelling and signal transduction. It has 2 isoforms produced by alternative splicing with the molecular weight of 80 kDa and 71 kDa.