验证数据展示
经过测试的应用
Positive WB detected in | mouse skeletal muscle tissue |
Positive IHC detected in | human urothelial carcinoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
Application | Dilution |
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Western Blot (WB) | WB : 1:200-1:1000 |
Immunohistochemistry (IHC) | IHC : 1:100-1:400 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
10415-1-AP targets Calpain 3 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
Tested Applications | WB, IHC, ELISA Application Description |
Tested Reactivity | human, mouse |
Immunogen | Calpain 3 fusion protein Ag0671 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | calpain 3, (p94) |
Synonyms | Calpain 3, calpain 3, (p94), Calpain L3, Calpain p94, CANP 3, CANP3, CANPL3, CAPN3, LGMD2, LGMD2A, nCL 1, NCL1, New calpain 1, p94 |
Calculated Molecular Weight | 94 kDa |
Observed Molecular Weight | 90-105 kDa, 55-60 kDa, 30-35 kDa |
GenBank Accession Number | BC003521 |
Gene Symbol | CAPN3 |
Gene ID (NCBI) | 825 |
RRID | AB_2069647 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | P20807 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Calpain 3 (CAPN3), a major intracellular protease, is a muscle-specific member of the calpain large subunit family that specifically binds to titin. CAPN3 plays a role in the dysferlin protein complex and that disruption of CAPN3 function may affect muscle membrane repair and remodeling. CAPN3 has some isoforms with MW of 94, 84, 93, 36, 18 kDa. CAPN3 autolysis generates a small N-terminal fragment of 34 kDa and a large C-terminal fragment whose size ranges from 55 to 60 kDa during self-processing (PMID: 9794799, 14645524).
实验方案
Product Specific Protocols | |
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WB protocol for Calpain 3 antibody 10415-1-AP | Download protocol |
IHC protocol for Calpain 3 antibody 10415-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |