Calpain 6 Polyclonal antibody
Calpain 6 Polyclonal Antibody for WB, IHC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (1)
Applications
WB, IHC, IP, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | human placenta tissue, mouse placenta tissue, A549 cells |
| Positive IP detected in | human placenta tissue |
| Positive IHC detected in | human intrahepatic cholangiocarcinoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
10120-1-AP targets Calpain 6 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human samples.
| Tested Applications | WB, IHC, IP, ELISA Application Description |
| Cited Applications | WB, IHC, IF |
| Tested Reactivity | human |
| Cited Reactivity | human, rat |
| Immunogen | Calpain 6 fusion protein Ag0160 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | calpain 6 |
| Synonyms | CAPN6, Calpain-like protease X-linked, Calpain-6, calpain6 |
| Calculated Molecular Weight | 75 kDa |
| Observed Molecular Weight | 66-75 kDa |
| GenBank Accession Number | BC000730 |
| Gene Symbol | Calpain 6 |
| Gene ID (NCBI) | 827 |
| RRID | AB_2290829 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9Y6Q1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Calpain 6 antibody 10120-1-AP | Download protocol |
| IHC protocol for Calpain 6 antibody 10120-1-AP | Download protocol |
| IP protocol for Calpain 6 antibody 10120-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Neuropathol Exp Neurol MicroRNA Alteration in Developing Rat Oligodendrocyte Precursor Cells Induced by Hypoxia-Ischemia. | ||
Am J Reprod Immunol The investigation of calpain in human placenta with fetal growth restriction. | ||
Amino Acids Advanced glycation end products promote the progression of chronic kidney diseases by targeting calpain 6 | ||
Cell Biosci Single-cell RNA sequencing analysis of the temporomandibular joint condyle in 3 and 4-month-old human embryos |
