验证数据展示
经过测试的应用
| Positive WB detected in | human placenta tissue, mouse placenta tissue, A549 cells |
| Positive IP detected in | human placenta tissue |
| Positive IHC detected in | human intrahepatic cholangiocarcinoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
10120-1-AP targets Calpain 6 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human |
| 文献引用反应性 | human, rat |
| 免疫原 | Calpain 6 fusion protein Ag0160 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | calpain 6 |
| 别名 | CAPN6, Calpain-like protease X-linked, Calpain-6, calpain6 |
| 计算分子量 | 75 kDa |
| 观测分子量 | 66-75 kDa |
| GenBank蛋白编号 | BC000730 |
| 基因名称 | Calpain 6 |
| Gene ID (NCBI) | 827 |
| RRID | AB_2290829 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9Y6Q1 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Calpain 6 antibody 10120-1-AP | Download protocol |
| IHC protocol for Calpain 6 antibody 10120-1-AP | Download protocol |
| IP protocol for Calpain 6 antibody 10120-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Neuropathol Exp Neurol MicroRNA Alteration in Developing Rat Oligodendrocyte Precursor Cells Induced by Hypoxia-Ischemia. | ||
Am J Reprod Immunol The investigation of calpain in human placenta with fetal growth restriction. | ||
Amino Acids Advanced glycation end products promote the progression of chronic kidney diseases by targeting calpain 6 | ||
Cell Biosci Single-cell RNA sequencing analysis of the temporomandibular joint condyle in 3 and 4-month-old human embryos |
