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Calpain 7 Polyclonal antibody

Calpain 7 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 13870-1-AP

Print datasheet

Synonyms

CAPN7, PalBH, EC:3.4.22.-, Calpain-7, CALPAIN7

验证数据展示

  • WB analysis of human heart using 13870-1-AP

    WB analysis of human heart using 13870-1-AP

    human heart tissue were subjected to SDS PAGE followed by western blot with 13870-1-AP (Calpain 7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary cancer using 13870-1-AP

    IHC staining of human ovary cancer using 13870-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 13870-1-AP (Calpain 7 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human intrahepatic cholangiocarcinoma using 13870-1-AP

    IHC staining of human intrahepatic cholangiocarcinoma using 13870-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 13870-1-AP (Calpain 7 antibody) at dilution of 1:50 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inhuman heart tissue
Positive IHC detected inhuman ovary cancer tissue, human intrahepatic cholangiocarcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13870-1-AP targets Calpain 7 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human
Immunogen Calpain 7 fusion protein Ag4820 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name calpain 7
Synonyms CAPN7, PalBH, EC:3.4.22.-, Calpain-7, CALPAIN7
Calculated Molecular Weight 93 kDa
Observed Molecular Weight 92 kDa
GenBank Accession NumberBC056202
Gene Symbol Calpain 7
Gene ID (NCBI) 23473
RRIDAB_2069823
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y6W3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for Calpain 7 antibody 13870-1-APDownload protocol
IHC protocol for Calpain 7 antibody 13870-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols