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验证数据展示

  • IHC staining of mouse stomach using 20971-1-AP

    IHC staining of mouse stomach using 20971-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 20971-1-AP (CAPN8 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human stomach cancer using 20971-1-AP

    IHC staining of human stomach cancer using 20971-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 20971-1-AP (CAPN8 antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

CAPN8 Polyclonal antibody

CAPN8 Polyclonal Antibody for IHC, ELISA
Cat No. 20971-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, ELISA

Synonyms

calpain 8, CAPN8, nCL 2, NCL2, New calpain 2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive IHC detected inmouse stomach tissue, human stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20971-1-AP targets CAPN8 in IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications IHC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name calpain 8
Synonyms calpain 8, CAPN8, nCL 2, NCL2, New calpain 2
Calculated Molecular Weight 79 kDa
GenBank Accession NumberNM_001143962
Gene Symbol CAPN8
Gene ID (NCBI) 388743
RRIDAB_3085628
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDA6NHC0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CAPN8 (Calpain-8) was found to be a significant risk factor for THCA (Thyroid cancer) with a markedly elevated level of mRNA and protein in tumor tissues. This potential oncogene could induce the activation of epithelial mesenchymal transition and E2F-targeted pathways (PMID:36389799).

实验方案

Product Specific Protocols
IHC protocol for CAPN8 antibody 20971-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols