验证数据展示
经过测试的应用
Positive IHC detected in | mouse stomach tissue, human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
Application | Dilution |
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Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
20971-1-AP targets CAPN8 in IHC, ELISA applications and shows reactivity with human, mouse samples.
Tested Applications | IHC, ELISA Application Description |
Tested Reactivity | human, mouse |
Immunogen | Peptide 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | calpain 8 |
Synonyms | calpain 8, CAPN8, nCL 2, NCL2, New calpain 2 |
Calculated Molecular Weight | 79 kDa |
GenBank Accession Number | NM_001143962 |
Gene Symbol | CAPN8 |
Gene ID (NCBI) | 388743 |
RRID | AB_3085628 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | A6NHC0 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CAPN8 (Calpain-8) was found to be a significant risk factor for THCA (Thyroid cancer) with a markedly elevated level of mRNA and protein in tumor tissues. This potential oncogene could induce the activation of epithelial mesenchymal transition and E2F-targeted pathways (PMID:36389799).
实验方案
Product Specific Protocols | |
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IHC protocol for CAPN8 antibody 20971-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |