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  • KD/KO Validated

CAPNS1 Polyclonal antibody

CAPNS1 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, WB,ELISA

Conjugate

Unconjugated

Cat No : 25057-1-AP

Print datasheet

Synonyms

30K, Calpain regulatory subunit, Calpain small subunit 1, calpain, small subunit 1, CALPAIN4, CANP, CANP small subunit, CANPS, CAPN4, CAPNS, CAPNS1, CDPS, CSS1



经过测试的应用

Positive WB detected inHEK-293 cells, A431 cells, mouse spleen tissue
Positive IHC detected inhuman kidney tissue, human stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy CAPNS1 Ready-To-Use IHC Kit. CAPNS1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 25057-1-AP is CAPNS1 Fusion Protein expressed in E. coli.

Tested Applications IHC, WB,ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Immunogen CAPNS1 fusion protein Ag21885 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name calpain, small subunit 1
Synonyms 30K, Calpain regulatory subunit, Calpain small subunit 1, calpain, small subunit 1, CALPAIN4, CANP, CANP small subunit, CANPS, CAPN4, CAPNS, CAPNS1, CDPS, CSS1
Calculated Molecular Weight 28 kDa
Observed Molecular Weight 28 kDa
GenBank Accession NumberBC011903
Gene Symbol CAPNS1
Gene ID (NCBI) 826
RRIDAB_2879876
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP04632
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for CAPNS1 antibody 25057-1-APDownload protocol
IHC protocol for CAPNS1 antibody 25057-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Cell Metab

High dietary fructose promotes hepatocellular carcinoma progression by enhancing O-GlcNAcylation via microbiota-derived acetate

Authors - Peng Zhou
humanWB

J Biol Chem

Calpain cleaves most components in the multiple aminoacyl-tRNA synthetase complex and affects their functions.

Authors - Hui-Yan Lei
  • KD Validated

Oncol Rep

Capn4 promotes epithelial-mesenchymal transition in human melanoma cells through activation of the Wnt/β-catenin pathway.

Authors - Enwen Wang
humanWB

Cell Rep

Epstein-Barr virus BNRF1 destabilizes SMC5/6 cohesin complexes to evade its restriction of replication compartments.

Authors - Stephanie Pei Tung Yiu