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Calpain S2 Polyclonal antibody

Calpain S2 Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Cat no : 15803-1-AP

Print datasheet

Synonyms

CAPNS2, CSS2, calpain, small subunit 2, Calpain small subunit 2, Calcium-dependent protease small subunit 2

验证数据展示

  • WB analysis of mouse skin using 15803-1-AP

    WB analysis of mouse skin using 15803-1-AP

    mouse skin tissue were subjected to SDS PAGE followed by western blot with 15803-1-AP (Calpain S2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse skin using 15803-1-AP

    IP experiment of mouse skin using 15803-1-AP

    IP result of anti-Calpain S2 (IP:15803-1-AP, 4ug; Detection:15803-1-AP 1:300) with mouse skin tissue lysate 2000ug.

  • IHC staining of human skin cancer using 15803-1-AP

    IHC staining of human skin cancer using 15803-1-AP

    Immunohistochemical analysis of paraffin-embedded skin cancer slide using 15803-1-AP (Calpain S2 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human skin cancer using 15803-1-AP

    IHC staining of human skin cancer using 15803-1-AP

    Immunohistochemical analysis of paraffin-embedded skin cancer slide using 15803-1-AP (Calpain S2 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inmouse skin tissue, rat skin tissue
Positive IP detected inmouse skin tissue
Positive IHC detected inhuman skin cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15803-1-AP targets Calpain S2 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IP, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen Calpain S2 fusion protein Ag8534 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name calpain, small subunit 2
Synonyms CAPNS2, CSS2, calpain, small subunit 2, Calpain small subunit 2, Calcium-dependent protease small subunit 2
Calculated Molecular Weight 248 aa, 28 kDa
Observed Molecular Weight 28 kDa
GenBank Accession NumberBC005397
Gene Symbol Calpain S2
Gene ID (NCBI) 84290
RRIDAB_2259580
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96L46
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Calpain S2, also known as calpain-2, is a member of the calpain family of calcium-dependent proteases that play crucial roles in various cellular processes.

实验方案

Product Specific Protocols
WB protocol for Calpain S2 antibody 15803-1-APDownload protocol
IHC protocol for Calpain S2 antibody 15803-1-APDownload protocol
IP protocol for Calpain S2 antibody 15803-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols