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验证数据展示

  • WB analysis of mouse skin using 15803-1-AP

    WB analysis of mouse skin using 15803-1-AP

    mouse skin tissue were subjected to SDS PAGE followed by western blot with 15803-1-AP (Calpain S2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse skin using 15803-1-AP

    IP experiment of mouse skin using 15803-1-AP

    IP result of anti-Calpain S2 (IP:15803-1-AP, 4ug; Detection:15803-1-AP 1:300) with mouse skin tissue lysate 2000ug.

  • IHC staining of human skin cancer using 15803-1-AP

    IHC staining of human skin cancer using 15803-1-AP

    Immunohistochemical analysis of paraffin-embedded skin cancer slide using 15803-1-AP (Calpain S2 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human skin cancer using 15803-1-AP

    IHC staining of human skin cancer using 15803-1-AP

    Immunohistochemical analysis of paraffin-embedded skin cancer slide using 15803-1-AP (Calpain S2 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Calpain S2 Polyclonal antibody

Calpain S2 Polyclonal Antibody for WB, IHC, IP, ELISA
Cat No. 15803-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IP, ELISA

别名

CAPNS2, CSS2, calpain, small subunit 2, Calpain small subunit 2, Calcium-dependent protease small subunit 2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse skin tissue, rat skin tissue
Positive IP detected inmouse skin tissue
Positive IHC detected inhuman skin cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15803-1-AP targets Calpain S2 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IP, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 Calpain S2 fusion protein Ag8534 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 calpain, small subunit 2
别名 CAPNS2, CSS2, calpain, small subunit 2, Calpain small subunit 2, Calcium-dependent protease small subunit 2
计算分子量 248 aa, 28 kDa
观测分子量 28 kDa
GenBank蛋白编号BC005397
基因名称 Calpain S2
Gene ID (NCBI) 84290
RRIDAB_2259580
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96L46
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Calpain S2, also known as calpain-2, is a member of the calpain family of calcium-dependent proteases that play crucial roles in various cellular processes.

实验方案

Product Specific Protocols
WB protocol for Calpain S2 antibody 15803-1-APDownload protocol
IHC protocol for Calpain S2 antibody 15803-1-APDownload protocol
IP protocol for Calpain S2 antibody 15803-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols