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验证数据展示

  • WB analysis of Y79 using 20766-1-AP

    WB analysis of Y79 using 20766-1-AP

    Y79 cells were subjected to SDS PAGE followed by western blot with 20766-1-AP (CAPRIN2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IP experiment of SH-SY5Y using 20766-1-AP

    IP experiment of SH-SY5Y using 20766-1-AP

    IP result of anti-CAPRIN2 (IP:20766-1-AP, 4ug; Detection:20766-1-AP 1:300) with SH-SY5Y cells lysate 3000ug.

  • IF Staining of HepG2 using 20766-1-AP

    IF Staining of HepG2 using 20766-1-AP

    Immunofluorescent analysis of HepG2 cells using 20766-1-AP (CAPRIN2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

  • Featured Product
  • KD/KO验证

CAPRIN2 Polyclonal antibody

CAPRIN2 Polyclonal Antibody for WB, IF/ICC, IP, ELISA
Cat No. 20766-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, IP, ELISA and More (2)

别名

Gastric cancer multidrug resistance-associated protein, EEG1, Cytoplasmic activation/proliferation-associated protein 2, Caprin-2, C1QDC1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inY79 cells, SH-SY5Y cells, HEK-293 cells
Positive IP detected inSH-SY5Y cells
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy CAPRIN2 Ready-To-Use IHC Kit. CAPRIN2 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20766-1-AP targets CAPRIN2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, IP, ELISA Application Description
文献引用应用WB, IHC, IF
经测试反应性 human, mouse, rat
文献引用反应性mouse, rat
免疫原 CAPRIN2 fusion protein Ag14745 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 caprin family member 2
别名 Gastric cancer multidrug resistance-associated protein, EEG1, Cytoplasmic activation/proliferation-associated protein 2, Caprin-2, C1QDC1
计算分子量 1127 aa, 126 kDa
观测分子量 126-150 kDa
GenBank蛋白编号BC007520
基因名称 CAPRIN2
Gene ID (NCBI) 65981
RRIDAB_10695763
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ6IMN6
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for CAPRIN2 antibody 20766-1-APDownload protocol
IF protocol for CAPRIN2 antibody 20766-1-APDownload protocol
IP protocol for CAPRIN2 antibody 20766-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
ratIHC

Neuroendocrinology

Transcriptional and post-transcriptional regulation of oxytocin and vasopressin gene expression by CREB3L1 and CAPRIN2.

Authors - Soledad Bárez-López
View Article
mouseWB, IF

Dev Dyn

Deficiency of the RNA binding protein Caprin2 causes lens defects and features of Peters anomaly.

Authors - Soma Dash
  • KO Validated
View Article