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  • Featured Product
  • KD/KO Validated

CAPRIN2 Polyclonal antibody

CAPRIN2 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, IP, ELISA and More (2)

Conjugate

Unconjugated

Cat No : 20766-1-AP

Print datasheet

Synonyms

Gastric cancer multidrug resistance-associated protein, EEG1, Cytoplasmic activation/proliferation-associated protein 2, Caprin-2, C1QDC1

验证数据展示

  • WB analysis of Y79 using 20766-1-AP

    WB analysis of Y79 using 20766-1-AP

    Y79 cells were subjected to SDS PAGE followed by western blot with 20766-1-AP (CAPRIN2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IP experiment of SH-SY5Y using 20766-1-AP

    IP experiment of SH-SY5Y using 20766-1-AP

    IP result of anti-CAPRIN2 (IP:20766-1-AP, 4ug; Detection:20766-1-AP 1:300) with SH-SY5Y cells lysate 3000ug.

  • IF Staining of HepG2 using 20766-1-AP

    IF Staining of HepG2 using 20766-1-AP

    Immunofluorescent analysis of HepG2 cells using 20766-1-AP (CAPRIN2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inY79 cells, SH-SY5Y cells, HEK-293 cells
Positive IP detected inSH-SY5Y cells
Positive IF/ICC detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20766-1-AP targets CAPRIN2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IF/ICC, IP, ELISA Application Description
Cited ApplicationsWB, IHC, IF
Tested Reactivity human, mouse, rat
Cited Reactivitymouse, rat
Immunogen CAPRIN2 fusion protein Ag14745 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name caprin family member 2
Synonyms Gastric cancer multidrug resistance-associated protein, EEG1, Cytoplasmic activation/proliferation-associated protein 2, Caprin-2, C1QDC1
Calculated Molecular Weight 1127 aa, 126 kDa
Observed Molecular Weight 126-150 kDa
GenBank Accession NumberBC007520
Gene Symbol CAPRIN2
Gene ID (NCBI) 65981
RRIDAB_10695763
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ6IMN6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for CAPRIN2 antibody 20766-1-APDownload protocol
IF protocol for CAPRIN2 antibody 20766-1-APDownload protocol
IP protocol for CAPRIN2 antibody 20766-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
ratIHC

Neuroendocrinology

Transcriptional and post-transcriptional regulation of oxytocin and vasopressin gene expression by CREB3L1 and CAPRIN2.

Authors - Soledad Bárez-López
View Article
mouseWB, IF

Dev Dyn

Deficiency of the RNA binding protein Caprin2 causes lens defects and features of Peters anomaly.

Authors - Soma Dash
  • KO Validated
View Article