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验证数据展示

  • WB analysis of human spleen using 16926-1-AP

    WB analysis of human spleen using 16926-1-AP

    human spleen tissue were subjected to SDS PAGE followed by western blot with 16926-1-AP (Calcyphosine antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • WB analysis using 16926-1-AP

    WB analysis using 16926-1-AP

    Human placenta tissue were subjected to SDS PAGE followed by western blot with 16926-1-AP (Calcyphosine antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IHC staining of human oesophagus cancer using 16926-1-AP

    IHC staining of human oesophagus cancer using 16926-1-AP

    Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 16926-1-AP (Calcyphosine antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human oesophagus cancer using 16926-1-AP

    IHC staining of human oesophagus cancer using 16926-1-AP

    Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 16926-1-AP (Calcyphosine antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Calcyphosine Polyclonal antibody

Calcyphosine Polyclonal Antibody for WB, IHC, ELISA
Cat No. 16926-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

Calcyphosin, calcyphosine, CAPS, CAPS1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inhuman placenta tissue, human spleen tissue, human colon tissue
Positive IHC detected inhuman oesophagus cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16926-1-AP targets Calcyphosine in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原 Calcyphosine fusion protein Ag10330 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 calcyphosine
别名 Calcyphosin, calcyphosine, CAPS, CAPS1
计算分子量 189 aa, 21 kDa
观测分子量 21 kDa
GenBank蛋白编号BC080599
基因名称 calcyphosine
Gene ID (NCBI) 828
RRIDAB_2070024
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ13938
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

calcyphosine is a calcium-binding protein, which may play a role in the regulation of ion transport. Calcyphosine (CAPS) was initially identified in the canine thyroid but has also been detected in humans and other mammals. CAPS synthesis is increased by thyroid‐stimulating hormone and cAMP analogs that promote cell proliferation and maintain cell differentiation. Recently, CAPS was found to be involved in many kinds of tumor.

实验方案

Product Specific Protocols
WB protocol for Calcyphosine antibody 16926-1-APDownload protocol
IHC protocol for Calcyphosine antibody 16926-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols