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验证数据展示

  • WB analysis of human heart using 55145-1-AP

    WB analysis of human heart using 55145-1-AP

    human heart tissue were subjected to SDS PAGE followed by western blot with 55145-1-AP (CAPZA1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 55145-1-AP

    IHC staining of human colon cancer using 55145-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 55145-1-AP (CAPZA1 Antibody) at dilution of 1:50 (under 10x lens).

    Ready-to-use IHC kits available with CAPZA1 antibody included
  • IHC staining of human colon cancer using 55145-1-AP

    IHC staining of human colon cancer using 55145-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 55145-1-AP (CAPZA1 Antibody) at dilution of 1:50 (under 40x lens).

    Ready-to-use IHC kits available with CAPZA1 antibody included

CAPZA1 Polyclonal antibody

CAPZA1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 55145-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

CAPPA1, CAPZ, CapZ alpha 1, CAPZA1, CAZ1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inhuman heart tissue, mouse brain tissue, mouse heart tissue
Positive IHC detected inhuman colon cancer tissue, human stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy CAPZA1 Ready-To-Use IHC Kit. CAPZA1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

55145-1-AP targets CAPZA1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 capping protein (actin filament) muscle Z-line, alpha 1
别名 CAPPA1, CAPZ, CapZ alpha 1, CAPZA1, CAZ1
计算分子量 33 kDa
观测分子量 34-40 kDa
GenBank蛋白编号NM_006135
基因名称 CAPZA1
Gene ID (NCBI) 829
RRIDAB_11182940
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP52907
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CAPZA1 belongs to the F-actin-capping protein alpha subunit family. F-actin-capping proteins bind in a Ca2+-independent manner to the fast growing ends of actin filaments (barbed end) thereby blocking the exchange of subunits at these ends. Unlike other capping proteins (such as gelsolin and severin), these proteins do not sever actin filaments. The potential for CAPZ is a therapeutic target in the management of heart failure. Future research is required to determine the mechanisms by which changes in CAPZ impact myocardial function and intracellular signalling, and to develop feasible strategies that can manipulate CAPZ in the intact heart. (PMID: 15614336 ) This antibody is specific to CAPZA1.

实验方案

Product Specific Protocols
WB protocol for CAPZA1 antibody 55145-1-APDownload protocol
IHC protocol for CAPZA1 antibody 55145-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols