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CAPZA1 Polyclonal antibody

CAPZA1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 55145-1-AP

Print datasheet

Synonyms

CAPPA1, CAPZ, CapZ alpha 1, CAPZA1, CAZ1

验证数据展示

  • WB analysis of human heart using 55145-1-AP

    WB analysis of human heart using 55145-1-AP

    human heart tissue were subjected to SDS PAGE followed by western blot with 55145-1-AP (CAPZA1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 55145-1-AP

    IHC staining of human colon cancer using 55145-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 55145-1-AP (CAPZA1 Antibody) at dilution of 1:50 (under 10x lens).

    Ready-to-use IHC kits available with CAPZA1 antibody included
  • IHC staining of human colon cancer using 55145-1-AP

    IHC staining of human colon cancer using 55145-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 55145-1-AP (CAPZA1 Antibody) at dilution of 1:50 (under 40x lens).

    Ready-to-use IHC kits available with CAPZA1 antibody included

经过测试的应用

Positive WB detected inhuman heart tissue, mouse brain tissue, mouse heart tissue
Positive IHC detected inhuman colon cancer tissue, human stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy CAPZA1 Ready-To-Use IHC Kit. CAPZA1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

55145-1-AP targets CAPZA1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name capping protein (actin filament) muscle Z-line, alpha 1
Synonyms CAPPA1, CAPZ, CapZ alpha 1, CAPZA1, CAZ1
Calculated Molecular Weight 33 kDa
Observed Molecular Weight 34-40 kDa
GenBank Accession NumberNM_006135
Gene Symbol CAPZA1
Gene ID (NCBI) 829
RRIDAB_11182940
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP52907
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CAPZA1 belongs to the F-actin-capping protein alpha subunit family. F-actin-capping proteins bind in a Ca2+-independent manner to the fast growing ends of actin filaments (barbed end) thereby blocking the exchange of subunits at these ends. Unlike other capping proteins (such as gelsolin and severin), these proteins do not sever actin filaments. The potential for CAPZ is a therapeutic target in the management of heart failure. Future research is required to determine the mechanisms by which changes in CAPZ impact myocardial function and intracellular signalling, and to develop feasible strategies that can manipulate CAPZ in the intact heart. (PMID: 15614336 ) This antibody is specific to CAPZA1.

实验方案

Product Specific Protocols
WB protocol for CAPZA1 antibody 55145-1-APDownload protocol
IHC protocol for CAPZA1 antibody 55145-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols