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  • Featured Product
  • KD/KO Validated

CAPZA1 Monoclonal antibody, PBS Only

CAPZA1 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

10H1F9

Cat no : 66066-1-PBS

Print datasheet

Synonyms

capzα1, F-actin-capping protein subunit alpha-1, CapZ alpha-1, CapZ alpha 1, CAPPA1

验证数据展示

  • WB analysis of HEK-293 using 66066-1-Ig (same clone as 66066-1-PBS)

    WB analysis of HEK-293 using 66066-1-Ig (same clone as 66066-1-PBS)

    WB result of CAPZA1 antibody (66066-1-Ig, 1:8000) with si-Control and si-CAPZA1 transfected HEK293 cells. This data was developed using the same antibody clone with 66066-1-PBS in a different storage buffer formulation.

  • WB analysis using 66066-1-Ig (same clone as 66066-1-PBS)

    WB analysis using 66066-1-Ig (same clone as 66066-1-PBS)

    Western blot analysis of CAPZA1 in various cell lines using Proteintech antibody 66066-1-Ig at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66066-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 66066-1-Ig (same clone as 66066-1-PBS)

    IF Staining of HeLa using 66066-1-Ig (same clone as 66066-1-PBS)

    Immunofluorescent analysis of Hela cells using 66066-1-Ig (CAPZA1 antibody) at dilution of 1:50 and Rhodamine-Goat anti-Rabbit IgG. This data was developed using the same antibody clone with 66066-1-PBS in a different storage buffer formulation.

  • IHC staining of human skin cancer using 66066-1-Ig (same clone as 66066-1-PBS)

    IHC staining of human skin cancer using 66066-1-Ig (same clone as 66066-1-PBS)

    Immunohistochemical analysis of paraffin-embedded skin cancer slide using 66066-1-Ig (CAPZA1 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66066-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CAPZA1 antibody included
  • IF Staining of HeLa using 66066-1-Ig (same clone as 66066-1-PBS)

    IF Staining of HeLa using 66066-1-Ig (same clone as 66066-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CAPZA1 antibody (66066-1-Ig, Clone: 10H1F9 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 66066-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy CAPZA1 Ready-To-Use IHC Kit. CAPZA1 primary antibody included.

产品信息

66066-1-PBS targets CAPZA1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen CAPZA1 fusion protein Ag18841 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name capping protein (actin filament) muscle Z-line, alpha 1
Synonyms capzα1, F-actin-capping protein subunit alpha-1, CapZ alpha-1, CapZ alpha 1, CAPPA1
Calculated Molecular Weight 286 aa, 33 kDa
Observed Molecular Weight 37 kDa
GenBank Accession NumberBC000144
Gene Symbol CAPZA1
Gene ID (NCBI) 829
RRIDAB_11042588
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP52907
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CAPZA1 (F-actin-capping protein subunit alpha-1) is a member of the F-actin capping protein alpha subunit family and regulates actin filament growth, stability and disassembly. Differential expression of CAPZA1 has been found in gastric cancer and melanoma, indicating its possible role in tumorigenesis. (21566537, 23545944)