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验证数据展示

  • WB analysis of HEK-293 using 66066-1-Ig (same clone as 66066-1-PBS)

    WB analysis of HEK-293 using 66066-1-Ig (same clone as 66066-1-PBS)

    WB result of CAPZA1 antibody (66066-1-Ig, 1:8000) with si-Control and si-CAPZA1 transfected HEK293 cells. This data was developed using the same antibody clone with 66066-1-PBS in a different storage buffer formulation.

  • WB analysis using 66066-1-Ig (same clone as 66066-1-PBS)

    WB analysis using 66066-1-Ig (same clone as 66066-1-PBS)

    Western blot analysis of CAPZA1 in various cell lines using Proteintech antibody 66066-1-Ig at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66066-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 66066-1-Ig (same clone as 66066-1-PBS)

    IF Staining of HeLa using 66066-1-Ig (same clone as 66066-1-PBS)

    Immunofluorescent analysis of Hela cells using 66066-1-Ig (CAPZA1 antibody) at dilution of 1:50 and Rhodamine-Goat anti-Rabbit IgG. This data was developed using the same antibody clone with 66066-1-PBS in a different storage buffer formulation.

  • IHC staining of human skin cancer using 66066-1-Ig (same clone as 66066-1-PBS)

    IHC staining of human skin cancer using 66066-1-Ig (same clone as 66066-1-PBS)

    Immunohistochemical analysis of paraffin-embedded skin cancer slide using 66066-1-Ig (CAPZA1 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66066-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CAPZA1 antibody included
  • IF Staining of HeLa using 66066-1-Ig (same clone as 66066-1-PBS)

    IF Staining of HeLa using 66066-1-Ig (same clone as 66066-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CAPZA1 antibody (66066-1-Ig, Clone: 10H1F9 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 66066-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

CAPZA1 Monoclonal antibody, PBS Only

CAPZA1 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 66066-1-PBS

产品说明书

CloneNo. 10H1F9

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

capzα1, F-actin-capping protein subunit alpha-1, CapZ alpha-1, CapZ alpha 1, CAPPA1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy CAPZA1 Ready-To-Use IHC Kit. CAPZA1 primary antibody included.

产品信息

66066-1-PBS targets CAPZA1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 CAPZA1 fusion protein Ag18841 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 capping protein (actin filament) muscle Z-line, alpha 1
别名 capzα1, F-actin-capping protein subunit alpha-1, CapZ alpha-1, CapZ alpha 1, CAPPA1
计算分子量 286 aa, 33 kDa
观测分子量 37 kDa
GenBank蛋白编号BC000144
基因名称 CAPZA1
Gene ID (NCBI) 829
RRIDAB_11042588
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP52907
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CAPZA1 (F-actin-capping protein subunit alpha-1) is a member of the F-actin capping protein alpha subunit family and regulates actin filament growth, stability and disassembly. Differential expression of CAPZA1 has been found in gastric cancer and melanoma, indicating its possible role in tumorigenesis. (21566537, 23545944)