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验证数据展示

  • WB analysis using 68190-1-Ig

    WB analysis using 68190-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68190-1-Ig (CAPZB antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

  • IHC staining of mouse testis using 68190-1-Ig

    IHC staining of mouse testis using 68190-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 68190-1-Ig (CAPZB antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CAPZB antibody included
  • FC experiment of Jurkat using 68190-1-Ig

    FC experiment of Jurkat using 68190-1-Ig

    1X10^6 Jurkat cells were intracellularly stained with 0.4 ug Anti-Human CAPZB (68190-1-Ig, Clone:1F9G2) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IF Staining of A431 using 68190-1-Ig

    IF Staining of A431 using 68190-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed A431 cells using CAPZB antibody (68190-1-Ig, Clone: 1F9G2 ) at dilution of 1:2000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

CAPZB Monoclonal antibody

CAPZB Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA
Cat No. 68190-1-Ig

产品说明书

CloneNo. 1F9G2

宿主/亚型

Mouse / IgG2a

种属反应性

Human, mouse, rat

应用

WB, IHC, IF/ICC, FC (Intra), ELISA

别名

F-actin-capping protein subunit beta, CapZbeta, CapZ beta, 1F9G2

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inA431 cells, HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells, RAW 264.7 cells
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells
Positive FC (Intra) detected inJurkat cells
Planning an IHC experiment? We recommend our IHCeasy CAPZB Ready-To-Use IHC Kit. CAPZB primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:1000-1:4000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68190-1-Ig targets CAPZB in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with Human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 Human, mouse, rat
免疫原 CAPZB fusion protein Ag19373 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 capping protein (actin filament) muscle Z-line, beta
别名 F-actin-capping protein subunit beta, CapZbeta, CapZ beta, 1F9G2
计算分子量 277 aa, 31 kDa
观测分子量31 kDa
GenBank蛋白编号BC109241
基因名称 CAPZB
Gene ID (NCBI) 832
RRIDAB_2935279
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP47756
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for CAPZB antibody 68190-1-IgDownload protocol
IHC protocol for CAPZB antibody 68190-1-IgDownload protocol
IF protocol for CAPZB antibody 68190-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols