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验证数据展示

  • WB analysis using 28483-1-AP

    WB analysis using 28483-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28483-1-AP (CARD11 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 28483-1-AP

    IHC staining of human colon cancer using 28483-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 28483-1-AP (CARD11 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human colon cancer using 28483-1-AP

    IHC staining of human colon cancer using 28483-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 28483-1-AP (CARD11 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

CARD11 Polyclonal antibody

CARD11 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 28483-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

Human and More (1)

应用

WB, IHC, ELISA

别名

BIMP3, CARD11, Carma 1, CARMA1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inK-562 cells, Raji cells
Positive IHC detected inhuman colon cancer tissue, human spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28483-1-AP targets CARD11 in WB, IHC, ELISA applications and shows reactivity with Human samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 Human
文献引用反应性human
免疫原 CARD11 fusion protein Ag29355 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 caspase recruitment domain family, member 11
别名 BIMP3, CARD11, Carma 1, CARMA1
计算分子量 1154 aa, 133 kDa
观测分子量 140-145 kDa
GenBank蛋白编号BC111719
基因名称 CARD11
Gene ID (NCBI) 84433
RRIDAB_2881153
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9BXL7
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CARD11 also named as ,BIMP3 or CARMA1, is a 1154 amino acid protein, which contains one PDZ domain, one guanylate kinase-like domain, and one CARD domain. CARD11 localizes in the cytoplasm and co-localized with DPP4 in membrane raft. CARD11 is detected in adult peripheral blood leukocytes, thymus, spleen and liver. CARD11 also is found in CARD11 promyelocytic leukemia HL-60 cells, chronic myelogenous leukemia K-562 cells, Burkitt's lymphoma Raji cells and colorectal adenocarcinoma SW480 cells. CARD11 is involved in the co-stimulatory signal essential for T-cell receptor mediated T-cell activation.

实验方案

Product Specific Protocols
WB protocol for CARD11 antibody 28483-1-APDownload protocol
IHC protocol for CARD11 antibody 28483-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

J Pathol Clin Res

Somatic mutations in four novel genes contribute to homologous recombination deficiency in breast cancer: a real-world clinical tumor sequencing study

Authors - Yongsheng Huang
  • KD Validated
View Article