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CARD11 Polyclonal antibody

CARD11 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human and More (1)

Applications

IHC, WB,ELISA

Conjugate

Unconjugated

Cat No : 28483-1-AP

Print datasheet

Synonyms

BIMP3, CARD11, Carma 1, CARMA1

验证数据展示

  • WB analysis using 28483-1-AP

    WB analysis using 28483-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28483-1-AP (CARD11 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 28483-1-AP

    IHC staining of human colon cancer using 28483-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 28483-1-AP (CARD11 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human colon cancer using 28483-1-AP

    IHC staining of human colon cancer using 28483-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 28483-1-AP (CARD11 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inK-562 cells, Raji cells
Positive IHC detected inhuman colon cancer tissue, human spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28483-1-AP targets CARD11 in WB, IHC, ELISA applications and shows reactivity with Human samples.

Tested Applications IHC, WB,ELISA Application Description
Cited ApplicationsWB
Tested Reactivity Human
Cited Reactivityhuman
Immunogen CARD11 fusion protein Ag29355 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name caspase recruitment domain family, member 11
Synonyms BIMP3, CARD11, Carma 1, CARMA1
Calculated Molecular Weight 1154 aa, 133 kDa
Observed Molecular Weight 140-145 kDa
GenBank Accession NumberBC111719
Gene Symbol CARD11
Gene ID (NCBI) 84433
RRIDAB_2881153
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BXL7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CARD11 also named as ,BIMP3 or CARMA1, is a 1154 amino acid protein, which contains one PDZ domain, one guanylate kinase-like domain, and one CARD domain. CARD11 localizes in the cytoplasm and co-localized with DPP4 in membrane raft. CARD11 is detected in adult peripheral blood leukocytes, thymus, spleen and liver. CARD11 also is found in CARD11 promyelocytic leukemia HL-60 cells, chronic myelogenous leukemia K-562 cells, Burkitt's lymphoma Raji cells and colorectal adenocarcinoma SW480 cells. CARD11 is involved in the co-stimulatory signal essential for T-cell receptor mediated T-cell activation.

实验方案

Product Specific Protocols
WB protocol for CARD11 antibody 28483-1-APDownload protocol
IHC protocol for CARD11 antibody 28483-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

J Pathol Clin Res

Somatic mutations in four novel genes contribute to homologous recombination deficiency in breast cancer: a real-world clinical tumor sequencing study

Authors - Yongsheng Huang
  • KD Validated
View Article