• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 14516-1-AP

    WB analysis using 14516-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14516-1-AP (CARD8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human prostate cancer using 14516-1-AP

    IHC staining of human prostate cancer using 14516-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 14516-1-AP (CARD8 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CARD8 antibody included
  • IHC staining of human prostate cancer using 14516-1-AP

    IHC staining of human prostate cancer using 14516-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 14516-1-AP (CARD8 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CARD8 antibody included
  • IHC staining of human tonsillitis using 14516-1-AP

    IHC staining of human tonsillitis using 14516-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 14516-1-AP (CARD8 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CARD8 antibody included

CARD8 Polyclonal antibody

CARD8 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 14516-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

DACAR, Caspase recruitment domain-containing protein 8, N-terminus, Caspase recruitment domain-containing protein 8, C-terminus, Caspase recruitment domain-containing protein 8, CARD-inhibitor of NF-kappa-B-activating ligand

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inA549 cells, HUVEC cells, THP-1 cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy CARD8 Ready-To-Use IHC Kit. CARD8 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14516-1-AP targets CARD8 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原 CARD8 fusion protein Ag5975 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 caspase recruitment domain family, member 8
别名 DACAR, Caspase recruitment domain-containing protein 8, N-terminus, Caspase recruitment domain-containing protein 8, C-terminus, Caspase recruitment domain-containing protein 8, CARD-inhibitor of NF-kappa-B-activating ligand
计算分子量61 kDa, 49 kDa
观测分子量61 kDa, 49 kDa
GenBank蛋白编号BC056891
基因名称 CARD8
Gene ID (NCBI) 22900
RRIDAB_2068262
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9Y2G2
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CARD8 belongs to the caspase recruitment domain (CARD)-containing family of proteins, which are involved in pathways leading to activation of caspases or nuclear factor kappa-B (NFKB). CARD8 may be a component of the inflammasome, a protein complex that plays a role in the activation of proinflammatory caspases. It is thought that this protein acts as an adaptor molecule that negatively regulates NFKB activation, CASP1-dependent IL1B secretion, and apoptosis. Polymorphisms in this gene may be associated with a susceptibility to rheumatoid arthritis.

实验方案

Product Specific Protocols
WB protocol for CARD8 antibody 14516-1-APDownload protocol
IHC protocol for CARD8 antibody 14516-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols