验证数据展示
经过测试的应用
| Positive WB detected in | HL-60 cells, THP-1 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
10669-1-AP targets CARD9 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB, IP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | CARD9 fusion protein Ag1057 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | caspase recruitment domain family, member 9 |
| 别名 | hCARD9, Caspase recruitment domain-containing protein 9 |
| 计算分子量 | 62 kDa |
| 观测分子量 | 55-62 kDa |
| GenBank蛋白编号 | BC008877 |
| 基因名称 | CARD9 |
| Gene ID (NCBI) | 64170 |
| RRID | AB_2228323 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9H257 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CARD9, a member of the caspase recruitment domain (CARD) protein family, plays an important regulatory role in cell apoptosis. Defects in CARD9 are the cause of familial candidiasis type 2 (CANDF2) characterized by impaired clearance of fungal infections and results in colonization and infections of the mucosa or skin, predominantly with Candida albicans. CARD9 is highly expressed in spleen and regulate activation of NF-kappa-B by interaction with postive regulator of apoptosis BCL10 via the CARD domain.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CARD9 antibody 10669-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Blood Invasive fungal infection and impaired neutrophil killing in human CARD9 deficiency. | ||
PLoS Pathog CARD9-Dependent Neutrophil Recruitment Protects against Fungal Invasion of the Central Nervous System. | ||
J Clin Immunol Inherited CARD9 Deficiency in a Child with Invasive Disease Due to Exophiala dermatitidis and Two Older but Asymptomatic Siblings. | ||
Transl Stroke Res Inhibition of Dectin-1 Ameliorates Neuroinflammation by Regulating Microglia/Macrophage Phenotype After Intracerebral Hemorrhage in Mice. | ||
Plast Reconstr Surg Chronic Candida albicans Meningitis in a 4-Year-Old Girl with a Homozygous Mutation in the CARD9 Gene (Q295X). | ||
Front Immunol TREM1 Regulates Neuroinflammatory Injury by Modulate Proinflammatory Subtype Transition of Microglia and Formation of Neutrophil Extracellular Traps via Interaction With SYK in Experimental Subarachnoid Hemorrhage. |