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  • KD/KO Validated

CARM1 Polyclonal antibody

CARM1 Polyclonal Antibody for IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, monkey

Applications

IHC, IF/ICC, ELISA and More (4)

Conjugate

Unconjugated

Cat no : 55246-1-AP

Print datasheet

Synonyms

CARM1, PRMT4



经过测试的应用

Positive IHC detected inhuman breast cancer tissue, human cervical cancer tissue, human malignant melanoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inNIH/3T3 cells
Planning an IHC experiment? We recommend our IHCeasy CARM1 Ready-To-Use IHC Kit. CARM1 primary antibody included.

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

55246-1-AP targets CARM1 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat, monkey samples.

Tested Applications IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB, IP, IF, IHC, CoIP
Tested Reactivity human, mouse, rat, monkey
Cited Reactivityhuman, mouse
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name coactivator-associated arginine methyltransferase 1
Synonyms CARM1, PRMT4
Calculated Molecular Weight 66 kDa
Observed Molecular Weight 63-66 kDa, 55 kDa
GenBank Accession NumberNM_199141
Gene Symbol CARM1
Gene ID (NCBI) 10498
RRIDAB_10863291
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ86X55
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

背景介绍

CARM1, also named as PRMT4, belongs to the protein arginine N-methyltransferase family. It is a dual functional coregulator that facilitates transcription initiation by methylation of Arg17 and Arg26 of histone H3 and also dictates the subsequent coactivator complex disassembly by methylation of the steroid receptor coactivator family coactivators and p300/cAMP-response element-binding protein. CARM1 functions as a coactivator for many nuclear receptors, such as oestrogen receptor, androgen receptor, thyroid receptor and farnesoid X-receptor. It also coactivates other transcription factors such as myocyte enhancer factor 2C (MEF2C), β-catenin, p53, nuclear factor (NF)-kB and the cAMP-responsive element-binding factor. The enzymatic activity and coactivator function of CARM1 has been found to be inactivated through phosphorylation at a conserved serine residue at mitosis stage. This antibody was generated against a synthetic peptide corresponding to a fragment of human CARM1. It is expected to specifically recognize the CRAM1. In certain type of cells, like Hela, double bands can be detected with this antibody. This may due to the additional PTM sites in cells themselves.

实验方案

Product Specific Protocols
WB protocol for CARM1 antibody 55246-1-APDownload protocol
IHC protocol for CARM1 antibody 55246-1-APDownload protocol
IF protocol for CARM1 antibody 55246-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

J Med Chem

Discovery of First-in-Class Dual PARP and EZH2 Inhibitors for Triple-Negative Breast Cancer with Wild-Type BRCA

Authors - Limei Ji
humanWB

Eur J Med Chem

Discovery of precision targeting EZH2 degraders for triple-negative breast cancer.

Authors - Cheng Wang
mouseWB

Front Cell Dev Biol

Osteocytes Enhance Osteogenesis by Autophagy-Mediated FGF23 Secretion Under Mechanical Tension.

Authors - Huiyue Xu
humanWB,CoIP

Cancer Cell Int

CDCA4 suppresses epithelial-mesenchymal transtion (EMT) and metastasis in Non-small cell lung cancer through modulating autophagy.

Authors - Chenxin Xu
  • KD Validated
humanWB

RSC Adv

Mechanistic insights into geniposide regulation of bile salt export pump (BSEP) expression

Authors - Guixin Wu
mouseIF

J Reprod Immunol

Poly(I:C) exposure during in vitro fertilization disrupts first cleavage of mouse embryos and subsequent blastocyst development.

Authors - Zhicheng Wang