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验证数据展示

  • WB analysis of MCF-7 using 27133-1-AP

    WB analysis of MCF-7 using 27133-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 27133-1-AP (CARMIL antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IP experiment of MCF-7 using 27133-1-AP

    IP experiment of MCF-7 using 27133-1-AP

    IP result of anti-CARMIL (IP:27133-1-AP, 4ug; Detection:27133-1-AP 1:1000) with MCF-7 cells lysate 2800 ug.

  • IHC staining of human colon using 27133-1-AP

    IHC staining of human colon using 27133-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 27133-1-AP (CARMIL antibody at dilution of 1:200 (under 10x lens).

  • IHC staining of human colon using 27133-1-AP

    IHC staining of human colon using 27133-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 27133-1-AP (CARMIL antibody at dilution of 1:200 (under 40x lens).

CARMIL Polyclonal antibody

CARMIL Polyclonal Antibody for WB, IP, IHC, ELISA
Cat No. 27133-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IP, IHC, ELISA

别名

CARMIL, CARMIL homolog, CARMIL1a, LRRC16, LRRC16A

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inMCF-7 cells, HepG2 cells, HeLa cells
Positive IP detected inMCF-7 cells
Positive IHC detected inhuman colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IHCSee 1 publications below

产品信息

27133-1-AP targets CARMIL in WB, IP, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IP, IHC, ELISA Application Description
文献引用应用IHC
经测试反应性 human
文献引用反应性human
免疫原 CARMIL fusion protein Ag25876 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 leucine rich repeat containing 16A
别名 CARMIL, CARMIL homolog, CARMIL1a, LRRC16, LRRC16A
观测分子量 152 kDa
GenBank蛋白编号BC140770
基因名称 CARMIL1
Gene ID (NCBI) 55604
RRIDAB_2880768
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ5VZK9
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for CARMIL antibody 27133-1-APDownload protocol
IHC protocol for CARMIL antibody 27133-1-APDownload protocol
IP protocol for CARMIL antibody 27133-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

Int Immunopharmacol

CARMIL1 regulates liver cancer cell proliferation by activating the ERK/mTOR pathway through the TRIM27/p53 axis

Authors - Yuzhen Ge
View Article