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  • Featured Product
  • KD/KO Validated

CARS Polyclonal antibody

CARS Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 15296-1-AP

Print datasheet

Synonyms

Cysteinyl-tRNA synthetase, cysteinyl tRNA synthetase, Cysteine--tRNA ligase, cytoplasmic, Cysteine tRNA ligase, CYSRS

验证数据展示

  • WB analysis using 15296-1-AP

    WB analysis using 15296-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15296-1-AP (CARS antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 15296-1-AP

    IHC staining of mouse brain using 15296-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15296-1-AP (CARS antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 15296-1-AP

    IHC staining of mouse brain using 15296-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15296-1-AP (CARS antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of MCF-7 using 15296-1-AP

    IF Staining of MCF-7 using 15296-1-AP

    Immunofluorescent analysis of MCF-7 cells, using CARS antibody 15296-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

经过测试的应用

Positive WB detected inA549 cells, HeLa cells
Positive IHC detected inmouse brain tissue, human pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15296-1-AP targets CARS in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB, IP
Tested Reactivity human, mouse
Cited Reactivityhuman
Immunogen CARS fusion protein Ag7546 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name cysteinyl-tRNA synthetase
Synonyms Cysteinyl-tRNA synthetase, cysteinyl tRNA synthetase, Cysteine--tRNA ligase, cytoplasmic, Cysteine tRNA ligase, CYSRS
Calculated Molecular Weight 89 kDa
Observed Molecular Weight 70-85 kDa
GenBank Accession NumberBC002880
Gene Symbol CARS
Gene ID (NCBI) 833
RRIDAB_2068455
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP49589
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for CARS antibody 15296-1-APDownload protocol
IHC protocol for CARS antibody 15296-1-APDownload protocol
IF protocol for CARS antibody 15296-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

EMBO J

CARS senses cysteine deprivation to activate AMPK for cell survival.

Authors - Mengqiu Yuan
  • KD Validated
View Article
humanWB,IP

Ann Rheum Dis

Mass spectrometry-based identification of new anti-Ly and known antisynthetase autoantibodies

Authors - Jean-Baptiste Vulsteke
View Article