MLN51/CASC3 Polyclonal antibody
MLN51/CASC3 Polyclonal Antibody for IF/ICC, IHC, WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
IF/ICC, IHC, WB,ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | A2780 cells, MCF-7 cells, SKOV-3 cells |
| Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HepG2 cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF) | IF : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 2 publications below |
| IF | See 1 publications below |
产品信息
The immunogen of 18047-1-AP is MLN51/CASC3 Fusion Protein expressed in E. coli.
| Tested Applications | IF/ICC, IHC, WB,ELISA Application Description |
| Cited Applications | WB, IF |
| Tested Reactivity | human |
| Cited Reactivity | human |
| Immunogen | MLN51/CASC3 fusion protein Ag12694 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | cancer susceptibility candidate 3 |
| Synonyms | BTZ, CASC3, MLN 51, MLN51, Protein barentsz, Protein CASC3 |
| Calculated Molecular Weight | 703 aa, 76 kDa |
| Observed Molecular Weight | 115-120 kDa |
| GenBank Accession Number | BC050526 |
| Gene Symbol | CASC3 |
| Gene ID (NCBI) | 22794 |
| RRID | AB_10640534 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O15234 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CASC3(cancer susceptibility candidate 3) is also named as MLN51 and belongs to the CASC3 family. It is induced by CSF2, and is important in the proliferation of RA fibroblast-like synoviocytes in the pathogenesis of rheumatoid arthritis (RA)(PMID:17101062). It is a nucleocytoplasmic protein containing, within its aminoterminal half, a coiled-coil domain followed by two nuclear localization signals (NLSs) responsible for nuclear localization and, in its carboxyl-terminal half, a nuclear export signal(NES) that mediates cytoplasmic retention. It possesses many potential phosphorylation sites and is found to be phosphorylated in both normal and cancer-derived cells.(PMID:17652158).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for MLN51/CASC3 antibody 18047-1-AP | Download protocol |
| IHC protocol for MLN51/CASC3 antibody 18047-1-AP | Download protocol |
| IF protocol for MLN51/CASC3 antibody 18047-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Mol Cell Identification of a microRNA that activates gene expression by repressing nonsense-mediated RNA decay. | ||
