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Caspase 9/p35/p10 Polyclonal antibody

Caspase 9/p35/p10 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 23821-1-AP

Print datasheet

Synonyms

验证数据展示

  • WB analysis of HeLa using 23821-1-AP

    WB analysis of HeLa using 23821-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 23821-1-AP (CASP9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human pancreas using 23821-1-AP

    IHC staining of human pancreas using 23821-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas slide using 23821-1-AP (CASP9 Antibody) at dilution of 1:50.

  • IHC staining of human pancreas using 23821-1-AP

    IHC staining of human pancreas using 23821-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas slide using 23821-1-AP (CASP9 Antibody) at dilution of 1:50.

  • IF Staining of HUVEC using 23821-1-AP

    IF Staining of HUVEC using 23821-1-AP

    Immunofluorescent analysis of HUVEC cells using 23821-1-AP (Caspase 9/p35/p10 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

经过测试的应用

Positive WB detected inHeLa cells,
Positive IHC detected inhuman pancreas tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHUVEC cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

23821-1-AP targets Caspase 9/p35/p10 in WB, IF, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IF, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivitymouse
Immunogen Caspase 9/p35/p10 fusion protein Ag20813 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name caspase 9, apoptosis-related cysteine peptidase
Synonyms
Calculated Molecular Weight 46 kDa
Observed Molecular Weight 46 kDa
GenBank Accession NumberBC002452
Gene Symbol Caspase 9
Gene ID (NCBI) 842
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP55211
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Caspase 9, apoptosis-related cysteine protease (CASP9,synonyms: MCH6, APAF3, APAF-3, ICE-LAP6, CASPASE-9c)is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce 2 subunits, large and small, that dimerize to form the active enzyme. CASP9 is processed by caspase APAF1; this step is thought to be one of the earliest in the caspase activation cascade

发表文章

SpeciesApplicationTitle
mouseWB

J Cell Physiol

Alpha-Lipoic Acid Promotes Osteoblastic Formation in H2 O2 -Treated MC3T3-E1 Cells and Prevents Bone Loss in Ovariectomized Rats.

Authors - Chao Fu
View Article