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验证数据展示

  • WB analysis using 14534-1-AP

    WB analysis using 14534-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14534-1-AP (CASZ1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

CASZ1 Polyclonal antibody

CASZ1 Polyclonal Antibody for WB, ELISA
Cat No. 14534-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, ELISA

别名

Castor related protein, castor zinc finger 1, CASZ1, CST, SRG, Zinc finger protein 693, ZNF693

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inSMMC-7721 cells, C2C12 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14534-1-AP targets CASZ1 in WB, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human, mouse
免疫原 CASZ1 fusion protein Ag6035 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 castor zinc finger 1
别名 Castor related protein, castor zinc finger 1, CASZ1, CST, SRG, Zinc finger protein 693, ZNF693
计算分子量 190 kDa
观测分子量150 kDa, 250 kDa
GenBank蛋白编号BC051883
基因名称 CASZ1
Gene ID (NCBI) 54897
RRIDAB_3669188
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ86V15
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Zinc finger protein castor homolog 1 (CASZ1), also known as zinc finger protein 693 (ZNF693), belongs to the C2H2-type zinc finger protein family. CASZ1 is a transcription factor that is evolutionarily conserved and participates in multiple embryonic development and physiological processes. CASZ1 has been found to regulate aldosterone synthesis and repress the activation of aldosterone target genes, suggesting that it may participate in blood pressure regulation. CASZ1 is ubiquitously expressed in various embryonic tissues. Subcellularly, CASZ1 is usually localized in the cell nucleus, consistent with its role in transcription regulation (PMID: 37509718). CASZ1 has two isoforms namely CASZ1a (i.e., hcasz11, 190 kDa) and CASZ1b (i.e., hcasz5, 125 kDa). The observed molecular weights were 150 kDa and 250 kDa, respectively, consistent with descriptions in the literature (PMID: 32060262).

实验方案

Product Specific Protocols
WB protocol for CASZ1 antibody 14534-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols