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CBARA1 Polyclonal antibody

CBARA1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 25905-1-AP

Print datasheet

Synonyms

EFHA3, Calcium-binding atopy-related autoantigen 1, CALC, Atopy-related autoantigen CALC, Atopy related autoantigen CALC

验证数据展示

  • WB analysis using 25905-1-AP

    WB analysis using 25905-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25905-1-AP (CBARA1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary cancer using 25905-1-AP

    IHC staining of human ovary cancer using 25905-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 25905-1-AP (CBARA1 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inHeLa cells, mouse heart tissue, rat heart tissue
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25905-1-AP targets CBARA1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen CBARA1 fusion protein Ag23176 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name calcium binding atopy-related autoantigen 1
Synonyms EFHA3, Calcium-binding atopy-related autoantigen 1, CALC, Atopy-related autoantigen CALC, Atopy related autoantigen CALC
Calculated Molecular Weight 54 kDa
Observed Molecular Weight 45-50 kDa
GenBank Accession NumberBC004190
Gene Symbol CBARA1
Gene ID (NCBI) 10367
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BPX6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for CBARA1 antibody 25905-1-APDownload protocol
IHC protocol for CBARA1 antibody 25905-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols