验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, HeLa cells, mouse testis tissue |
| Positive IHC detected in | human gliomas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| ChIP | See 1 publications below |
产品信息
11336-1-AP targets CBFA2T2 in WB, IHC, chIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | chIP |
| 经测试反应性 | human, mouse, rat |
| 免疫原 | CBFA2T2 fusion protein Ag1880 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | core-binding factor, runt domain, alpha subunit 2; translocated to, 2 |
| 别名 | CBFA2T2, EHT, MTG8 like protein, MTG8 related protein 1, MTGR1, p85, Protein CBFA2T2, ZMYND3 |
| 计算分子量 | 604 aa, 67 kDa |
| 观测分子量 | 80 kDa |
| GenBank蛋白编号 | BC015066 |
| 基因名称 | CBFA2T2 |
| Gene ID (NCBI) | 9139 |
| RRID | AB_2070571 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O43439 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Core-binding factor, runt domain, alpha subunit 2, translocated to 2(CBFA2T2) belongs to a family of ubiquitously expressed transcriptional repressors that interact with transcription factors bound to promoters of target genes and with histone deacetylases (HDACs) critical for enforcement of repressor function.CBFA2T2, also named as MTGR1 or EHT, is an 85-kDa phosphoprotein. It can bind AML1-MTG8 fusion protein in acute myeloid leukemia. The binding leads to an enhancement of cell proliferation mediated in a murine myeloid model. The calculated molecular weight of CBFA2T2 is 67 kDa, but modified SMARCE1 is about 80 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CBFA2T2 antibody 11336-1-AP | Download protocol |
| IHC protocol for CBFA2T2 antibody 11336-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
