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  • Featured Product
  • KD/KO Validated

CBFA2T3 Polyclonal antibody

CBFA2T3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA and More (2)

Conjugate

Unconjugated

Cat no : 17190-1-AP

Print datasheet

Synonyms

MTG8-related protein 2, MTG8 related protein 2, MTG16, hMTG16, ETO2

验证数据展示

  • WB analysis of Jurkat using 17190-1-AP

    WB analysis of Jurkat using 17190-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 17190-1-AP (CBFA2T3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of rat brain using 17190-1-AP

    IHC staining of rat brain using 17190-1-AP

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 17190-1-AP (CBFA2T3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inJurkat cells
Positive IHC detected inrat brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17190-1-AP targets CBFA2T3 in WB, IHC, IF, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB, IHC, IF, ChIP
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen CBFA2T3 fusion protein Ag9265 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name core-binding factor, runt domain, alpha subunit 2; translocated to, 3
Synonyms MTG8-related protein 2, MTG8 related protein 2, MTG16, hMTG16, ETO2
Calculated Molecular Weight 71 kDa
Observed Molecular Weight 62-65 kDa
GenBank Accession NumberBC062624
Gene Symbol CBFA2T3
Gene ID (NCBI) 863
RRIDAB_10859097
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO75081
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for CBFA2T3 antibody 17190-1-APDownload protocol
IHC protocol for CBFA2T3 antibody 17190-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB, IHC, IF

Oncotarget

miR-27a and miR-27a* contribute to metastatic properties of osteosarcoma cells.

Authors - Zaidoun Salah
View Article
human,mouseWB,IHC

JCI Insight

MTG16 is a tumor suppressor in colitis-associated carcinoma.

Authors - Elizabeth M McDonough
  • KO Validated
View Article
mouseWB,ChIP

J Biol Chem

Transcription factor Zbtb1 interacts with bridging factor Lmo2 and maintains the T-lineage differentiation capacity of lymphoid progenitor cells

Authors - Maria Koizumi
View Article

Leukemia

High caspase 3 and vulnerability to dual BCL2 family inhibition define ETO2::GLIS2 pediatric leukemia

Authors - Zakia Aid
View Article