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CBLB Polyclonal antibody

CBLB Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 12781-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, ELISA and More (2)

Casitas B-lineage lymphoma proto-oncogene b, E3 ubiquitin-protein ligase CBL-B, EC:2.3.2.27, Nbla00127, RING finger protein 56

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inHEK-293 cells, A549 cells
Positive IP detected inHEK-293 cells
Positive IHC detected inmouse colon tissue, human kidney tissue, human lung tissue, human placenta tissue, human spleen tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12781-1-AP targets CBLB in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
文献引用应用WB, IHC, IF, IP, CoIP
经测试反应性 human, mouse, rat
文献引用反应性human, mouse, rat
免疫原 CBLB fusion protein Ag3491 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 Cas-Br-M (murine) ecotropic retroviral transforming sequence b
别名 Casitas B-lineage lymphoma proto-oncogene b, E3 ubiquitin-protein ligase CBL-B, EC:2.3.2.27, Nbla00127, RING finger protein 56
计算分子量 982 aa, 109 kDa
观测分子量 110-130 kDa
GenBank蛋白编号BC032851
基因名称 CBLB
Gene ID (NCBI) 868
RRIDAB_2275326
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ13191
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CBLB(E3 ubiquitin-protein ligase CBL-B) is also named RNF56 and plays a necessary role in preventing the inappropriate activation of NF-B in response to lipopolysaccharide and polymicrobial sepsis. CBLB can be phosphorylated on tyrosine and serine residues upon TCR or BCR activation, and various types of cell stimulation. It also has a crucial role in preventing chronic inflammation and autoimmunity. This protein has 3 isoforms produced by alternative splicing 109, 90, and 86 kDa, and observed molecular weight at 110-130 kDa.

实验方案

Product Specific Protocols
WB protocol for CBLB antibody 12781-1-APDownload protocol
IHC protocol for CBLB antibody 12781-1-APDownload protocol
IF protocol for CBLB antibody 12781-1-APDownload protocol
IP protocol for CBLB antibody 12781-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

J Exp Med

Cbl-b deficiency prevents functional but not phenotypic T cell anergy.

Authors - Trang T T Nguyen
humanWB

Anal Chem

Ionic Liquid-Based Extraction System for In-Depth Analysis of Membrane Protein Complexes.

Authors - Hongwei Chu
ratIHC

Sci Signal

Ubiquitination and functional modification of GluN2B subunit-containing NMDA receptors by Cbl-b in the spinal cord dorsal horn.

Authors - Zi-Yang Zhang
humanFC

J Immunol

A Multiple Sclerosis-Associated Variant of CBLB Links Genetic Risk with Type I IFN Function.

Authors - Klarissa Hanja Stürner
mouseWB,IF

J Cell Mol Med

NR4A1 counteracts JNK activation incurred by ER stress or ROS in pancreatic β-cells for protection.

Authors - Ze-Qing Pu
mouseWB

Biomolecules

miR-27b-3p Attenuates Muscle Atrophy by Targeting Cbl-b in Skeletal Muscles.

Authors - Xin Yang
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