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CBLL1 Polyclonal antibody

CBLL1 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

IHC, WB,ELISA

Conjugate

Unconjugated

Cat No : 29219-1-AP

Print datasheet

Synonyms

c Cbl like protein 1, CBLL1, FLJ23109, HAKAI, RING finger protein 188, RNF188

验证数据展示

  • WB analysis using 29219-1-AP

    WB analysis using 29219-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29219-1-AP (CBLL1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of human lung cancer using 29219-1-AP

    IHC staining of human lung cancer using 29219-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 29219-1-AP (CBLL1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inA549 cells, HeLa cells, NCI-H1299 cells
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29219-1-AP targets CBLL1 in WB, IHC,ELISA applications and shows reactivity with Human samples.

Tested Applications IHC, WB,ELISA
Tested Reactivity Human
Immunogen CBLL1 fusion protein Ag30900 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name Cas-Br-M (murine) ecotropic retroviral transforming sequence-like 1
Synonyms c Cbl like protein 1, CBLL1, FLJ23109, HAKAI, RING finger protein 188, RNF188
Calculated Molecular Weight 491 aa, 55 kDa
Observed Molecular Weight 55-60 kDa
GenBank Accession NumberBC027460
Gene Symbol CBLL1
Gene ID (NCBI) 79872
RRIDAB_2918251
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ75N03
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

背景介绍

Cbl proto‐oncogene E3 ubiquitin protein ligase‐like 1, RNF188, Hakai (CBLL1) is an evolutionarily conserved E3 ubiquitin ligase containing a RING‐finger domain. CBLL1 contains a typical RING‐finger, short pTyr‐binding domain, and proline‐rich domain. CBLL1 expression is upregulated in human colon and gastric cancer tissues, and CBLL1 has been reported to induce anchorage‐dependent cell growth. CBLL1 immunostaining was observed in both the nuclei and cytoplasm of cancer cells. (PMID: 31124298, PMID: 31646569, PMID: 21191016)

实验方案

Product Specific Protocols
WB protocol for CBLL1 antibody 29219-1-APDownload protocol
IHC protocol for CBLL1 antibody 29219-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols