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验证数据展示

  • WB analysis of SH-SY5Y using 18207-1-AP

    WB analysis of SH-SY5Y using 18207-1-AP

    WB result of CBLN1 antibody (18207-1-AP; 1:400; incubated at room temperature for 1.5 hours) with sh-Control and sh-CBLN1 transfected SH-SY5Y cells.

  • WB analysis using 18207-1-AP

    WB analysis using 18207-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 18207-1-AP (CBLN1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Featured Product
  • KD/KO验证

CBLN1 Polyclonal antibody

CBLN1 Polyclonal Antibody for WB, ELISA
Cat No. 18207-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, ELISA

别名

CBLN1, CER [des Ser1] cerebellin, Cerebellin 1, cerebellin 1 precursor, Precerebellin

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inSH-SY5Y cells, U-87 MG cells, Neuro-2a cells, mouse brain tissue, rat brain tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

18207-1-AP targets CBLN1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 CBLN1 fusion protein Ag12835 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 cerebellin 1 precursor
别名 CBLN1, CER [des Ser1] cerebellin, Cerebellin 1, cerebellin 1 precursor, Precerebellin
计算分子量 193 aa, 21 kDa
观测分子量 50 kDa
GenBank蛋白编号BC093692
基因名称 CBLN1
Gene ID (NCBI) 869
RRIDAB_3669294
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP23435
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CBLN1 (Cerebellin-1) is also named as Precerebellin. CBLN1 is a newly identified synaptic organizer belonging to the C1q family. Cbln1 was recently identified as the missing ligand for the orphan glutamate receptor δ2 (GluD2) (PMID: 21342763). In the cerebellum, Cbln1 is produced and secreted from granule cells and works as a strong synapse organizer between Purkinje cells and parallel fibers, the axons of the granule cells (PMID: 24607546). The glycosylation modification of CBLN1 resulted in a larger molecular weight.

实验方案

Product Specific Protocols
WB protocol for CBLN1 antibody 18207-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols