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验证数据展示

  • WB analysis of mouse cerebellum using 20559-1-AP

    WB analysis of mouse cerebellum using 20559-1-AP

    mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 20559-1-AP (CBLN4 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human cerebellum using 20559-1-AP

    IHC staining of human cerebellum using 20559-1-AP

    Immunohistochemical analysis of paraffin-embedded human cerebellum using 20559-1-AP (CBLN4 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human cerebellum using 20559-1-AP

    IHC staining of human cerebellum using 20559-1-AP

    Immunohistochemical analysis of paraffin-embedded human cerebellum using 20559-1-AP (CBLN4 antibody) at dilution of 1:50 (under 40x lens).

CBLN4 Polyclonal antibody

CBLN4 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 20559-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

CBLN4, CBLNL1, Cerebellin 4, cerebellin 4 precursor, Cerebellin like glycoprotein 1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse cerebellum tissue, mouse brain tissue, mouse kidney tissue
Positive IHC detected inhuman cerebellum tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20559-1-AP targets CBLN4 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 cerebellin 4 precursor
别名 CBLN4, CBLNL1, Cerebellin 4, cerebellin 4 precursor, Cerebellin like glycoprotein 1
计算分子量 22 kDa
观测分子量 22 kDa
GenBank蛋白编号NM_080617
基因名称 CBLN4
Gene ID (NCBI) 140689
RRIDAB_2878701
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NTU7
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CBLN4 may be involved in synaptic functions in the CNS. CBLN4 can enable ER export and secretion of CBLN3. The antibody has no cross reaction to CBLN1, 2, or 3.

实验方案

Product Specific Protocols
WB protocol for CBLN4 antibody 20559-1-APDownload protocol
IHC protocol for CBLN4 antibody 20559-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols