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验证数据展示

  • WB analysis of mouse cerebellum using 20559-1-AP

    WB analysis of mouse cerebellum using 20559-1-AP

    mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 20559-1-AP (CBLN4 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human cerebellum using 20559-1-AP

    IHC staining of human cerebellum using 20559-1-AP

    Immunohistochemical analysis of paraffin-embedded human cerebellum using 20559-1-AP (CBLN4 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human cerebellum using 20559-1-AP

    IHC staining of human cerebellum using 20559-1-AP

    Immunohistochemical analysis of paraffin-embedded human cerebellum using 20559-1-AP (CBLN4 antibody) at dilution of 1:50 (under 40x lens).

CBLN4 Polyclonal antibody

CBLN4 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 20559-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

CBLN4, CBLNL1, Cerebellin 4, cerebellin 4 precursor, Cerebellin like glycoprotein 1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inmouse cerebellum tissue, mouse brain tissue, mouse kidney tissue
Positive IHC detected inhuman cerebellum tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20559-1-AP targets CBLN4 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name cerebellin 4 precursor
Synonyms CBLN4, CBLNL1, Cerebellin 4, cerebellin 4 precursor, Cerebellin like glycoprotein 1
Calculated Molecular Weight 22 kDa
Observed Molecular Weight 22 kDa
GenBank Accession NumberNM_080617
Gene Symbol CBLN4
Gene ID (NCBI) 140689
RRIDAB_2878701
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NTU7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CBLN4 may be involved in synaptic functions in the CNS. CBLN4 can enable ER export and secretion of CBLN3. The antibody has no cross reaction to CBLN1, 2, or 3.

实验方案

Product Specific Protocols
WB protocol for CBLN4 antibody 20559-1-APDownload protocol
IHC protocol for CBLN4 antibody 20559-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols