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CBP80 Recombinant antibody

CBP80 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, rat

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240012G8

Cat no : 83212-4-RR

Print datasheet

Synonyms

CBP80, NCBP, NCBP 80 kDa subunit, NCBP1

验证数据展示

  • WB analysis using 83212-4-RR

    WB analysis using 83212-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83212-4-RR (CBP80 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • FC experiment of HepG2 using 83212-4-RR

    FC experiment of HepG2 using 83212-4-RR

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug CBP80 Recombinant antibody (83212-4-RR, Clone:240012G8) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IF Staining of HepG2 using 83212-4-RR

    IF Staining of HepG2 using 83212-4-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CBP80 antibody (83212-4-RR, Clone: 240012G8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 83212-4-RR

    Affinity and Kinetic Characterization of 83212-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83212-4-RR against Human CBP80 were performed. The affinity constant is 0.131 nM.

经过测试的应用

Positive WB detected inJurkat cells, HeLa cells, HepG2 cells, A431 cells, NCI-H1299 cells, PC-12 cells, HEK-293 cells
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells
Positive FC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy NCBP1 Ready-To-Use IHC Kit. NCBP1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83212-4-RR targets CBP80 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with Human, rat samples.

Tested Applications WB, IF/ICC, FC (Intra), ELISA Application Description
Tested Reactivity Human, rat
Immunogen CBP80 fusion protein Ag0496 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name nuclear cap binding protein subunit 1, 80kDa
Synonyms CBP80, NCBP, NCBP 80 kDa subunit, NCBP1
Calculated Molecular Weight 92 kDa
Observed Molecular Weight 80 kDa
GenBank Accession NumberBC001450
Gene Symbol NCBP1
Gene ID (NCBI) 4686
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ09161
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Nuclear cap binding protein subunit 1, 80kDa (NCBP1, synonyms: NCBP, CBP80) is a subunit of a cap-binding protein complex (CBC) that consists of a CBP20 subunit, which binds the cap, and a NCBP1 subunit, which ensures high-affinity cap binding. The CBC complex may play a role in pre-mRNA recognition and splicing.

实验方案

Product Specific Protocols
WB protocol for CBP80 antibody 83212-4-RRDownload protocol
IF protocol for CBP80 antibody 83212-4-RRDownload protocol
FC protocol for CBP80 antibody 83212-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols