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NCBP1/CBP80 Recombinant antibody

NCBP1/CBP80 Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240012G8

Cat no : 83212-4-RR

Print datasheet

Synonyms

NCBP1, NCBP 80 kDa subunit, NCBP, CBP80, 80 kDa nuclear cap-binding protein

验证数据展示

  • WB analysis using 83212-4-RR

    WB analysis using 83212-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83212-4-RR (CBP80 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary cancer using 83212-4-RR

    IHC staining of human ovary cancer using 83212-4-RR

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 83212-4-RR (NCBP1/CBP80 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with NCBP1 antibody included
  • FC experiment of HepG2 using 83212-4-RR

    FC experiment of HepG2 using 83212-4-RR

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug CBP80 Recombinant antibody (83212-4-RR, Clone:240012G8) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IF Staining of HepG2 using 83212-4-RR

    IF Staining of HepG2 using 83212-4-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CBP80 antibody (83212-4-RR, Clone: 240012G8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 83212-4-RR

    Affinity and Kinetic Characterization of 83212-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83212-4-RR against Human CBP80 were performed. The affinity constant is 0.131 nM.

经过测试的应用

Positive WB detected inJurkat cells, HeLa cells, HepG2 cells, A431 cells, NCI-H1299 cells, PC-12 cells, HEK-293 cells
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy NCBP1 Ready-To-Use IHC Kit. NCBP1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83212-4-RR targets NCBP1/CBP80 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, rat samples.

Tested Applications WB, IHC, IF/ICC, FC (Intra), ELISA Application Description
Tested Reactivity human, rat
Immunogen NCBP1/CBP80 fusion protein Ag0496 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name nuclear cap binding protein subunit 1, 80kDa
Synonyms NCBP1, NCBP 80 kDa subunit, NCBP, CBP80, 80 kDa nuclear cap-binding protein
Calculated Molecular Weight 92 kDa
Observed Molecular Weight 80 kDa
GenBank Accession NumberBC001450
Gene Symbol NCBP1
Gene ID (NCBI) 4686
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ09161
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Nuclear cap binding protein subunit 1, 80kDa (NCBP1, synonyms: NCBP, CBP80) is a subunit of a cap-binding protein complex (CBC) that consists of a CBP20 subunit, which binds the cap, and a NCBP1 subunit, which ensures high-affinity cap binding. The CBC complex may play a role in pre-mRNA recognition and splicing.

实验方案

Product Specific Protocols
WB protocol for NCBP1/CBP80 antibody 83212-4-RRDownload protocol
IHC protocol for NCBP1/CBP80 antibody 83212-4-RRDownload protocol
IF protocol for NCBP1/CBP80 antibody 83212-4-RRDownload protocol
FC protocol for NCBP1/CBP80 antibody 83212-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols