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验证数据展示

  • WB analysis using 31279-1-AP

    WB analysis using 31279-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31279-1-AP (CBR1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IP experiment of HeLa using 31279-1-AP

    IP experiment of HeLa using 31279-1-AP

    IP result of anti-CBR1 (IP:31279-1-AP, 4ug; Detection:31279-1-AP 1:5000) with HeLa cells lysate 980 ug.

  • IF Staining of HeLa using 31279-1-AP

    IF Staining of HeLa using 31279-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CBR1 antibody (31279-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

CBR1 Polyclonal antibody

CBR1 Polyclonal Antibody for WB, IF/ICC, IP, ELISA
Cat No. 31279-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, rat

应用

WB, IF/ICC, IP, ELISA

别名

EC:1.1.1.189, EC:1.1.1.184, carbonyl reductase 1, Alcohol dehydrogenase [NAD(P)+] CBR1, 20-beta-hydroxysteroid dehydrogenase

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, H9C2 cells
Positive IP detected inHeLa cells
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31279-1-AP targets CBR1 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, rat samples.

经测试应用 WB, IF/ICC, IP, ELISA Application Description
经测试反应性 human, rat
免疫原 CBR1 fusion protein Ag34830 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 carbonyl reductase 1
别名 EC:1.1.1.189, EC:1.1.1.184, carbonyl reductase 1, Alcohol dehydrogenase [NAD(P)+] CBR1, 20-beta-hydroxysteroid dehydrogenase
计算分子量 30 kDa
观测分子量 30 kDa
GenBank蛋白编号BC002511
基因名称 CBR1
Gene ID (NCBI) 873
RRIDAB_3669926
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP16152
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CBR1 belongs to the short-chain dehydrogenases/reductases (SDR) family, which function as NADPH-dependent oxidoreductases having wide specificity for carbonyl compounds, such as quinones, prostaglandins, and various xenobiotics.

实验方案

Product Specific Protocols
WB protocol for CBR1 antibody 31279-1-APDownload protocol
IF protocol for CBR1 antibody 31279-1-APDownload protocol
IP protocol for CBR1 antibody 31279-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols