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验证数据展示

  • WB analysis of BxPC-3 using 13725-1-AP

    WB analysis of BxPC-3 using 13725-1-AP

    BxPC-3 cells were subjected to SDS PAGE followed by western blot with 13725-1-AP (CBR4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human hepatocirrhosis using 13725-1-AP

    IHC staining of human hepatocirrhosis using 13725-1-AP

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 13725-1-AP (CBR4 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human hepatocirrhosis using 13725-1-AP

    IHC staining of human hepatocirrhosis using 13725-1-AP

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 13725-1-AP (CBR4 antibody) at dilution of 1:50 (under 40x lens).

  • IP experiment of HepG2 using 13725-1-AP

    IP experiment of HepG2 using 13725-1-AP

    IP result of anti-CBR4 (IP:13725-1-AP, 4ug; Detection:13725-1-AP 1:300) with HepG2 cells lysate 3400ug.

CBR4 Polyclonal antibody

CBR4 Polyclonal Antibody for WB, IP, IHC, ELISA
Cat No. 13725-1-AP

Print datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Synonyms

carbonyl reductase 4, CBR4, Quinone reductase CBR4, SDR45C1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inBxPC-3 cells, HepG2 cells
Positive IP detected inHepG2 cells
Positive IHC detected inhuman hepatocirrhosis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13725-1-AP targets CBR4 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IP, IHC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen CBR4 fusion protein Ag4678 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name carbonyl reductase 4
Synonyms carbonyl reductase 4, CBR4, Quinone reductase CBR4, SDR45C1
Calculated Molecular Weight 237 aa, 25 kDa
Observed Molecular Weight 28 kDa
GenBank Accession NumberBC033650
Gene Symbol CBR4
Gene ID (NCBI) 84869
RRIDAB_10643240
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8N4T8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for CBR4 antibody 13725-1-APDownload protocol
IHC protocol for CBR4 antibody 13725-1-APDownload protocol
IP protocol for CBR4 antibody 13725-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols