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  • Featured Product
  • KD/KO Validated

CBX1 Polyclonal antibody

CBX1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 10241-2-AP

Print datasheet

Synonyms

HP1Hsbeta, HP1Hs beta, HP1 beta, Heterochromatin protein p25, Chromobox protein homolog 1

验证数据展示

  • WB analysis using 10241-2-AP

    WB analysis using 10241-2-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10241-2-AP (CBX1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IP experiment of HEK-293 using 10241-2-AP

    IP experiment of HEK-293 using 10241-2-AP

    IP result of anti-CBX1 (IP:10241-2-AP, 3ug; Detection:10241-2-AP 1:500) with HEK-293 cells lysate 1480ug.

  • IF Staining of HEK-293 using 10241-2-AP

    IF Staining of HEK-293 using 10241-2-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HEK-293 cells using 10241-2-AP (CBX1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

  • WB analysis of MCF-7 using 10241-2-AP

    WB analysis of MCF-7 using 10241-2-AP

    WB result of CBX1 antibody (10241-2-AP; 1:2400; incubated at room temperature for 1.5 hours) with sh-Control and sh-CBX1 transfected MCF-7 cells.

  • IHC staining of mouse testis using 10241-2-AP

    IHC staining of mouse testis using 10241-2-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 10241-2-AP (CBX1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CBX1 antibody included
  • IHC staining of mouse testis using 10241-2-AP

    IHC staining of mouse testis using 10241-2-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 10241-2-AP (CBX1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CBX1 antibody included
  • IHC staining of human colon cancer using 10241-2-AP

    IHC staining of human colon cancer using 10241-2-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10241-2-AP (CBX1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CBX1 antibody included
  • IHC staining of human colon cancer using 10241-2-AP

    IHC staining of human colon cancer using 10241-2-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10241-2-AP (CBX1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CBX1 antibody included
  • IHC staining of human lung cancer using 10241-2-AP

    IHC staining of human lung cancer using 10241-2-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10241-2-AP (CBX1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CBX1 antibody included
  • IHC staining of human lung cancer using 10241-2-AP

    IHC staining of human lung cancer using 10241-2-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10241-2-AP (CBX1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CBX1 antibody included
  • IHC staining of human ovary tumor using 10241-2-AP

    IHC staining of human ovary tumor using 10241-2-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 10241-2-AP (CBX1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CBX1 antibody included
  • IHC staining of human ovary tumor using 10241-2-AP

    IHC staining of human ovary tumor using 10241-2-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 10241-2-AP (CBX1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CBX1 antibody included
  • IHC staining of human stomach cancer using 10241-2-AP

    IHC staining of human stomach cancer using 10241-2-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 10241-2-AP (CBX1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CBX1 antibody included
  • IHC staining of human stomach cancer using 10241-2-AP

    IHC staining of human stomach cancer using 10241-2-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 10241-2-AP (CBX1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CBX1 antibody included

经过测试的应用

Positive WB detected inA431 cells, MCF-7 cells, HEK-293 cells, human brain tissue, NIH/3T3 cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman stomach cancer tissue, mouse testis tissue, human colon cancer tissue, human lung cancer tissue, human ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHEK-293 cells
Planning an IHC experiment? We recommend our IHCeasy CBX1 Ready-To-Use IHC Kit. CBX1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10241-2-AP targets CBX1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, IP, ELISA Application Description
Cited ApplicationsWB, IHC, IP, IF
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen CBX1 fusion protein Ag0328 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name chromobox homolog 1 (HP1 beta homolog Drosophila )
Synonyms HP1Hsbeta, HP1Hs beta, HP1 beta, Heterochromatin protein p25, Chromobox protein homolog 1
Calculated Molecular Weight 27 kDa
Observed Molecular Weight 27 kDa
GenBank Accession NumberBC002609
Gene Symbol CBX1
Gene ID (NCBI) 10951
RRIDAB_2228586
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP83916
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

One function of heterochromatin is the epigenetic silencing by sequestration of genes into transcriptionally repressed nuclear neighborhoods. Heterochromatin protein 1 (HP1) is a major component of heterochromatin and is a candidate for establishing and mediating gene silencing. This protein is expressed in all adult and embryonic tissues, as a homodimer.

实验方案

Product Specific Protocols
WB protocol for CBX1 antibody 10241-2-APDownload protocol
IHC protocol for CBX1 antibody 10241-2-APDownload protocol
IF protocol for CBX1 antibody 10241-2-APDownload protocol
IP protocol for CBX1 antibody 10241-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB,IP

J Exp Med

ZNF382 controls mouse neuropathic pain via silencer-based epigenetic inhibition of Cxcl13 in DRG neurons.

Authors - Longfei Ma
View Article
mouseWB

Nat Commun

The histone H3K9 methyltransferase SUV39H links SIRT1 repression to myocardial infarction.

Authors - Guang Yang
View Article
humanWB,IHC,IF

J Cell Biol

Induction of alternative lengthening of telomeres-associated PML bodies by p53/p21 requires HP1 proteins.

Authors - Jiang Wei-Qin WQ
  • KD Validated
View Article
humanWB

J Virol

PIWIL4 Maintains HIV-1 Latency by Enforcing Epigenetically Suppressive Modifications on the 5' Long Terminal Repeat.

Authors - Zhangping He
View Article
humanWB,IF

Front Genet

Identification and Validation of Chromobox Family Members as Potential Prognostic Biomarkers and Therapeutic Targets for Human Esophageal Cancer.

Authors - Xuefen Fang
View Article
humanWB,IHC,IP

Adv Sci (Weinh)

N6 -Methyladenosine-Modified CBX1 Regulates Nasopharyngeal Carcinoma Progression Through Heterochromatin Formation and STAT1 Activation

Authors - Yin Zhao
View Article