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验证数据展示

  • WB analysis using 31108-1-AP

    WB analysis using 31108-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31108-1-AP (CBX1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

CBX1 Polyclonal antibody

CBX1 Polyclonal Antibody for WB, ELISA
Cat No. 31108-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, ELISA

别名

HP1Hsbeta, HP1Hs beta, HP1 beta, Heterochromatin protein p25, Chromobox protein homolog 1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA431 cells, HEK-293 cells, MCF-7 cells, NIH/3T3 cells
Planning an IHC experiment? We recommend our IHCeasy CBX1 Ready-To-Use IHC Kit. CBX1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31108-1-AP targets CBX1 in WB, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human, mouse
免疫原 CBX1 fusion protein Ag34901 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 chromobox homolog 1 (HP1 beta homolog Drosophila )
别名 HP1Hsbeta, HP1Hs beta, HP1 beta, Heterochromatin protein p25, Chromobox protein homolog 1
计算分子量 27 kDa
观测分子量21-27 kDa
GenBank蛋白编号BC002609
基因名称 CBX1
Gene ID (NCBI) 10951
RRIDAB_3669859
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity Purification
UNIPROT IDP83916
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CBX1 (chromobox 1), also known as CBX. It is expected to be located in nucleus, the protein is expressed in all adult and embryonic tissues. It is a member of the heterochromatin protein family. CBX1 is enriched in the heterochromatin and associated with centromeres. The protein has a single N-terminal chromodomain which can bind to histone proteins via methylated lysine residues, and a C-terminal chromo shadow-domain (CSD) which is responsible for the homodimerization and interaction with a number of chromatin-associated nonhistone proteins. It may play an important role in the epigenetic control of chromatin structure and gene expression. The calculated molecular weight of CBX1 is 21 kDa.

实验方案

Product Specific Protocols
WB protocol for CBX1 antibody 31108-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols